Site-Specific Antibody Production

All custom modification antibody undergoes double affinity purification with modified and unmodified peptides.

PTMs (Post-Translational Modifications) are an important consideration in research. They contribute to a variety of biological activities and affect protein structures and cellular functions. Protein phosphorylation, methylation, and acetylation are the most common types of modifications of the more than 200 different attached modifications known to exist in vivo.

Site-specific antibodies have a number of potential applications: cell signaling, protein structure, function, development, maturation, localization, disease diagnostics, activity, etc. Over the years, ABclonal has developed thousands of custom site-specific antibodies.

Modification Types

ABclonal provides services on the following custom modification antibodies.

  • Phosphorylation: Citrullination, Prenylation, Glycosylation
  • Acetylation: Succinylation, Sumoylation, Biotinylation
  • Methylation: Alkylation, Tyrosine Sulfation, Polyglutamylation
  • Ubiquitination: Isoprenylation, Tyrosine Nitration, Other PTMs
  • Others: DNA methylation, RNA methylation, Panacetylation, non-histone modification, and other custom specific modified antibodies that are developed to meet your research needs.

Site-Specific Antibody Production Process

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5 mL per rabbit)
Primary Injection Day 1 Immunize with 0.50 mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (-25 mL per rabbit)
3rd Booster Day 42 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (total of -50 mL per rabbit)
ELISA Test Day 60 ELISA titration (results available online)
Verify disposition of rabbits
Decide to continue or terminate
Purification Optional 6-10 mg of purified antibodies
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