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All custom modification antibody undergoes double affinity purification with modified and unmodified peptides.
PTMs (Post-Translational Modifications) are an important consideration in research. They contribute to a variety of biological activities and affect protein structures and cellular functions. Protein phosphorylation, methylation, and acetylation are the most common types of modifications of the more than 200 different attached modifications known to exist in vivo.
Site-specific antibodies have a number of potential applications: cell signaling, protein structure, function, development, maturation, localization, disease diagnostics, activity, etc. Over the years, ABclonal has developed thousands of custom site-specific antibodies.
ABclonal provides services on the following custom modification antibodies.
| Procedure | Protocol Day | Description |
|---|---|---|
| Control Serum Collection | Day 0 | Pre-immune bleed (5 mL per rabbit) |
| Primary Injection | Day 1 | Immunize with 0.50 mg of antigen in CFA, 10 SQ sites |
| 1st Booster | Day 14 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| 2nd Booster | Day 28 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| Serum Collection | Day 35 | Bleed (-25 mL per rabbit) |
| 3rd Booster | Day 42 | Boost with 0.25 mg of antigen in IFA, 4 SQ sites |
| Serum Collection | Day 56, 58 | Two bleeds (total of -50 mL per rabbit) |
| ELISA Test | Day 60 | ELISA titration (results available online) Verify disposition of rabbits Decide to continue or terminate |
| Purification | Optional | 6-10 mg of purified antibodies |
