Rabbit Monoclonal Antibodies

The Rabbit-mAb™ 3.0 generation of Recombinant Rabbit Monoclonal Antibody Service is exclusively available from ABclonal. Rabbit-mAb™ employs innovative cell sorting, enriching, bioinformatics, and recombinant monoclonal antibody technologies. ABclonal selects the desired monoclonal antibody directly from B cells.

Our rabbit monoclonal technologies provide more candidates that are unreachable by traditional technologies - our in-house bioinformatics optimizes the selection, our new generation of gene synthesis solutions cuts down the cost of antibody engineering, and our XPromoter 2.0 can highly express recombinant antibodies.

ABclonal provides custom rabbit monoclonal antibodies with high specificity and sensitivity in multiple applications and manufactures cost-efficient large-scale antibodies.

Custom Services offer you:
  1. High-quality custom antibodies tailored to your applications
  2. Managed by PhD-level scientist
  3. Opportunities to perform sample testing at various stages
  4. Implemented in Cambridge, Massachusetts, not offshore
Timeline
Phase I: Immunization

Phase II: Cell Sorting, Screening, Sequencing

Phase III: Cloning, Expression, and Characterization

To work with us, please provide:
  1. The antigen information: ABclonal will produce the immunogens for you, such as peptides, recombinant proteins, etc.
  2. Prepared immunogens: such as peptides, proteins, small molecules, or other samples.
  3. Spleen and blood of an immunized rabbit: ABclonal will initiate the custom monoclonal antibody development.

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.

The rabbit immune system generates antibody diversity and optimizes affinity via different mechanisms than rodents. ABclonal provide high quality Rabbit-mAb™ 3.0 service with SMab™ platform(Single cell-based, Speedy platform delivers Superior MAb) to customers.

The Advantages and Applications of Rabbit mAb


Advantages

High affinity

10-10 - 10-12 M, 10-100 times high KD than mouse MAbs

Superior specificity

GALT and B cell maturation

Broad epitope recognitiony

Less immunodominance, more diversity

Good hapten recognition

Express groups 1-3 CD1 proteins

Great Stability

Extra disulfide bonds

One major IgG isotype

Applications

High Affinity Antibody

Modification-State Antibody

Anti-Idiotype Antibody

Antibody Pairing and ELISA Kit

Research and In Vitro Diagnostic

Able to differentiate between subtle structural variations

Fit for low-abundance biomarker quantification

Convenient for use with mouse proteins and tissues

The ABclonal Ra-mAb™ 3.0 Advantages
  1. No the traditional cell fusion step
  2. No stability issues associated with hybridomas
  3. Recombinant antibody ensures constant production
  4. Can provide both monoclonal and polyclonal antibodies
  5. Flexible optimization at each stage
  6. High and cost-efficient production

Timeline and deliverables for rabbit mAb


Overview of standard animal immunization schedules

Deliverables:

2-3mg of four different recombinant rabbit monoclonal antibodies(guarantee indirect ELISA)

Full-length antibody genes of selected

Clones in plasmid forma

Case Study 1: first rabbit MAbs against CCL18


only mouse MAbs with clear cross-reactivity or low affinity.

high homology among CCL18 and its CC family members

counter screen;

boost with non-homologous peptides

develop high affinity, specific rabbit MAbs against CCL18


  • 1200 single B cell clones
  • 119 positive clones (10%)
  • 26 selected clones
  • 24 pairs of MAb genes
  • 22 functional MAbs
  • 17 specific anti-CCL18 MAbs
  • 6 multi-assay Mabs for final delivery
    -Sandwich ELISA
    -Western Blot

Case study 2: efficient screening of anti-hapten rabbit MAbs


Rabbit is better than mouse in presenting nonpeptic haptens due to its expression of CD1 family.

only mouse MAbs with clear cross-reactivity or low affinity.


  • 1334 single B cell clones
  • 100 positive clones (7.4%)
  • 18 selected clones
  • 17 pairs of MAb genes
  • 16 functional MAbs

Case study 3: quick discovery of PDL1-blocking antibodies


ligand-receptor interaction

PK and PD studies

potential therapeutical targets


Overview of standard animal immunization schedules

Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.
  4. Our customer service is available 24/7.