ABclonal provides a customized rabbit recombinant monoclonal antibody service using our proprietary B cell cloning technology. This unique technology employs innovative cell sorting, enriching, bioinformatics and advanced cloning. It does not take the traditional approach of using the hybridoma method, hence avoiding the stability issue associated with hybridomas.
Our rabbit monoclonal technology has a high success rate towards difficult targets such as post-translational modification site, small molecule, and proteins with point mutation.
We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.
Overview of standard animal immunization schedules
100 µg of up to five different recombinant rabbit monoclonal antibodies (ELISA positive)
Full-length antibody genes of selected clone
Clones in plasmid format
Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.
Rabbits are better than mice in presenting nonpeptic haptens because of their expression of proteins in the CD1 family. For this project, results achieved include:
PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include: