Superior Antibody Development With the SMab™ Platform
ABclonal provides one-stop, comprehensive custom monoclonal antibody production services using our innovative SMab™ technology, the first antibody discovery platform using single primary B cell ex vivo expansion. Our technology employs cell sorting, enrichment, bioinformatics, and advanced cloning to avoid the stability issues associated with the traditional hybridoma approach. SMab™ provides valuable flexibility and high success rates in generating antibodies against difficult targets including post-translational modifications, small molecules, and can even differentiate between proteins with point mutations.
The ABclonal Advantage
SMab™: Advanced single B cell screening and cloning technology to enhance yield and specificity of monoclonal antibodies
10+ years of success with established and constantly improving technology
Streamlined process to reduce turnaround time and improve specificity and yields
US-based production and animal care facilities with no outsourcing
Ex vivo primary B cell expansion allows for more efficient screening of positive clones
IACUC approval for all animal-related processes
Linear expression module for accelerated vector construction
Rabbit Monoclonal Antibody Production
While SMab™ can accommodate any species, ABclonal recommends the use of rabbit as host due to the advantages conferred by the rabbit immune system. This includes the ability to recognize a wider range of diverse antigens as well as the ability to produce antibodies with greater affinity and specificity.
Advantages of Rabbit Antibodies
Higher affinity and superior specificity compared to mouse antibodies
Broad epitope recognition across large range of antigens, including small molecules and post-translational modifications
Accommodates myriad experimental applications, including but not limited to:
- Western blotting
- Immunohistochemistry
- Immunofluorescence with conventional or confocal microscopy
- Immunoprecipitation and chromatin IP
- Flow cytometry
Your One-Stop Shop for Your Ideal Antibody Reagent!
Build the optimal antibody to your desired target, leveraging our decade-long in-house expertise to manufacture the best possible reagent to produce consistent results in your experimental applications!
Service Highlights
Comprehensive
World-Class Service
Under the supervision of talented experts, ABclonal works closely with you from initial consultation and antigen design all the way through to final antibody production and validation.
Flexible Optimization
At All Stages
Adjust your antibody project on the fly at any stage of the process, including testing serum and purified antibody with your own samples and protocols.
Antibody Adjustments
and Accommodations
In addition to optimization, ABclonal can provide both polyclonal and monoclonal antibodies to the desired antigen.
Own Your Antibody
ABclonal claims no intellectual property for or derived from the resulting antibody product, and will provide all information associated with your custom antibody project.
We also provide comprehensive services for antibody cloning, engineering, expression, and humanization in addition to our standard antibody production service.
Rabbit Monoclonal Antibody Production Process
3-4 WEEKS
Antigen Preparation
- Antigen Analysis & Design
- Antigen Synthesis
- Sort Antigen-specific Cells
- Culturing
- Primary Screening
Using ELISA Or Other
Assays If Desired
- Antibody Gene Cloning
And Secondary Screening
- Construct Expression
Vectors
- Expression
- Purification
- Validation Assays
Deliverables:
100 µg of as many rabbit monoclonal antibodies clones as desired from project.
Full-length naturally-paired heavy and light chain antibody sequences.
Antibody genes cloned into expression vectors.
Be The Next Success Story
First Rabbit mAbs Against CCL18
Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.
- 1200 single B cell clones
- 119 positive clones (10%)
- 26 selected clones
- 24 pairs of mAb genes
- 22 functional mAbs
- 17 specific anti-CCL18 mAbs
- 6 multi-assay mAbs
Sandwish ELISA
WB
Affinity
Efficient Screening Of Anti-Hapten Rabbit mAbs
Rabbits are better than mice in presenting nonpeptic haptens because of their expression of proteins in the CD1 family. For this project, results achieved include:
- 1334 single B cell clones
- 100 positive clones (7.4%)
- 18 selected clones
- 17 pairs of mAb genes
- 16 functional mAbs
ELISA Results
ELISA Results
Quick Discovery Of PD-L1-Blocking Antibodies
PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include:
- 2304 single B cell clones
- 386 positive clones (16.8%)
- 19 strong PDL1-blocking clones (4.9%)