Rabbit Monoclonal Antibodies

ABclonal provides a customized rabbit recombinant monoclonal antibody service using our proprietary B cell cloning technology. This unique technology employs innovative cell sorting, enriching, bioinformatics and advanced cloning. It does not take the traditional approach of using the hybridoma method, hence avoiding the stability issue associated with hybridomas.

Our rabbit monoclonal technology has a high success rate towards difficult targets such as post-translational modification site, small molecule, and proteins with point mutation.

Our rabbit monoclonal service provides:
  • One-stop service – from consultation, antigen design and synthesis to antibody purification and validation.
  • Flexible optimization at each stage.
  • Both monoclonal antibodies and polyclonal antibodies if requested.
  • Claims no Intellectual Property for the resulting antibody product.
Advantages of Rabbit mAb:
  • High affinity
  • Superior specificity
  • Broad epitope recognition
  • Good recognition of small molecules and post translational modification sites
  • Great stability
  • Multiple applications, including IF, IHC, ChIP, ELISA, Flow, IP and WB
  • High antibody diversity
  • Perfect choice for testing rodent proteins and tissues
  • One major IgG isotype
Timeline
Phase I: Immunization

Phase II: Cell Sorting, Screening, Sequencing

Phase III: Cloning, Expression, and Characterization

To work with us, please provide:
  1. The antigen information: ABclonal will produce the immunogens for you, such as peptides, recombinant proteins, etc.
  2. Prepared immunogens: such as peptides, proteins, small molecules, or other samples.
  3. Spleen and blood of an immunized rabbit: ABclonal will initiate the custom monoclonal antibody development.

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.

Timeline and Deliverables for Rabbit mAb


Overview of standard animal immunization schedules

Deliverables:

2-3 mg of four different recombinant rabbit monoclonal antibodies (guarantees indirect ELISA)

Full-length antibody genes of selected clone

Clones in plasmid format

Case Study 1: First Rabbit mAbs Against CCL18


Only rabbit mAbs with clear cross-reactivity or low affinity

High homology among CCL18 and its CC family members

Counter screen

Boost with non-homologous peptides

Develop high-affinity, specific rabbit mAbs against CCL1


  • 1200 single B cell clones
  • 119 positive clones (10%)
  • 26 selected clones
  • 24 pairs of mAb genes
  • 22 functional mAbs
  • 17 specific anti-CCL18 mAbs
  • 6 multi-assay mAbs for final delivery
    ○ Sandwich ELISA
    ○ Western Blot

Case Study 2: Efficient Screening Of Anti-Hapten Rabbit mAbs


Rabbit is better than mouse in presenting nonpeptic haptens due to its expression of CD1 family.

Only rabbit mAbs with clear cross-reactivity or low affinity


  • 1334 single B cell clones
  • 100 positive clones (7.4%)
  • 18 selected clones
  • 17 pairs of mAb genes
  • 16 functional mAbs

Case Study 3: Quick Discovery Of PD-L1-Blocking Antibodies


Ligand-receptor interaction

PK and PD studies

Potential therapeutical targets


Overview of standard animal immunization schedules

Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.