ABclonal provides one-stop, comprehensive custom monoclonal antibody production services using our innovative SMab™ technology, the first antibody discovery platform using single primary B cell ex vivo expansion. Our technology employs cell sorting, enrichment, bioinformatics, and advanced cloning to avoid the stability issues associated with the traditional hybridoma approach. SMab™ provides valuable flexibility and high success rates in generating antibodies against difficult targets including post-translational modifications, small molecules, and can even differentiate between proteins with point mutations.
SMab™: Advanced single B cell screening and cloning technology to enhance yield and specificity of monoclonal antibodies
10+ years of success with established and constantly improving technology
Streamlined process to reduce turnaround time and improve specificity and yields
US-based production and animal care facilities with no outsourcing
Ex vivo primary B cell expansion allows for more efficient screening of positive clones
IACUC approval for all animal-related processes
Linear expression module for accelerated vector construction
While SMab™ can accommodate any species, ABclonal recommends the use of rabbit as host due to the advantages conferred by the rabbit immune system. This includes the ability to recognize a wider range of diverse antigens as well as the ability to produce antibodies with greater affinity and specificity.
The ABclonal Rabbit mAb Advantage
Higher affinity and superior specificity compared to mouse antibodies
Broad epitope recognition across large range of antigens, including small molecules and post-translational modifications
Utilizing our years of expertise in concert with unique artificial intelligence (AI)-assisted algorithm to balance affinity and immunogenicity in antigen design
Accommodates myriad experimental applications, including but not limited to:
Build the optimal antibody to your desired target, leveraging our decade-long in-house expertise to manufacture the best possible reagent to produce consistent results in your experimental applications!
Service Highlights
Comprehensive
World-Class Service
Under the supervision of talented experts, ABclonal works closely with you from initial consultation and antigen design all the way through to final antibody production and validation.
Flexible Optimization
At All Stages
Adjust your antibody project on the fly at any stage of the process, including testing serum and purified antibody with your own samples and protocols.
Antibody Adjustments
and Accommodations
In addition to optimization, ABclonal can provide both polyclonal and monoclonal antibodies to the desired antigen.
Own Your Antibody
ABclonal claims no intellectual property for or derived from the resulting antibody product, and will provide all information associated with your custom antibody project.
We also provide comprehensive services for antibody cloning, engineering, expression, and humanization in addition to our standard antibody production service.
Most rabbit monoclonal antibodies available in the current market face challenges such as having low affinity and exhibiting clear cross-reactivity. However, our client entrusted us to develop a high-affinity, specific rabbit monoclonal antibodies against CCL18 – a protein that is highly homologous with other members in the CC family. By using methods such as antigen-binding ELISA assay and using non-homologous peptides as boosts, the following results were achieved.
Sandwich ELISA
WB
Affinity
Rabbits are better than mice in presenting nonpeptic haptens because of their expression of proteins in the CD1 family. For this project, results achieved include:
ELISA Results
ELISA Results
PD1 is a popular target in life science research that involves in the ligand-receptor interaction. For this project, results achieved include:
Phosphorylation
Acetylation
Methylation
Other Unique PTMs
3-4 WEEKS
Antigen Preparation
6-9 WEEKS
Immunization
2-4 WEEKS
Screening
2-4 WEEKS
Production
Deliverables:
100 µg of as many rabbit monoclonal antibodies clones as desired from project.Full-length naturally-paired heavy and light chain antibody sequences.Antibody genes cloned into expression vectors.
