ABclonal provides a reliable and cost-efficient custom polyclonal antibody service in SPF rabbit, mouse, rat, goat, chicken and guinea pig for gene-specific detection reagents. Our polyclonal antibodies can be used in multiple applications, including Western blots, ELISA, and IHC. However, developing highly specific and sensitive polyclonal antibody production is challenging and requires expertise during every step. Our team utilizes state-of-the-art scientific advances and the best tools to provide the highest quality polyclonal antibody service available.

Packages


  • Protein antigen design and expression
  • Peptide design and synthesis
  • Customer supplied immunogen (protein or peptide)
  • Protein antigen preparation/Peptide-KLH conjugation
  • Immunization of 2 rabbits(or other animals)
  • ELISA titer test
  • Antigen affinity column purification

One-stop Service

Note
1. If the antigen is provided by the customer: 5 mg of peptide or 5 mg of protein, purity >90% is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.

The Host Species


ABclonal offers a variety of protocols (immunization schedules for rabbits, mouse, rat, goats, etc.) for preparing custom polyclonal antibodies.

Our scientists will help you select the best antibody service according to the host and your protein characteristic and research application.

Additional Services


4-WEEK EXTENSION
1 additional immunization and 2 blood collections for 80-100ml of antisera.
AFFINITY PURIFICATION
Affinity purification of 25ml of sera. Includes the affinity column.
ELISA
On any bleed or purified antibody according to customer request.
WESTERN BLOT
Assay of antisera or purified antibodies according to customer request.
IMMUNOHISTOCHEMISTRY
Assay of purified antibodies against tissue sections according to customer request.
PEPTIDE SYNTHESIS
Full range of custom peptide services available.
PROTEIN ANTIGENS
Full range of custom protein services available.

Professional antigen design(free)MORE


Proper design of a suitable antigen is the most critical step of getting an useful antibody.

High-quality antigen preparationMORE


According to the customers’ demand and the target characteristics, our experts choose the best way to get the best immunogen.

Protein antigen

We experiment with a number of conditions to improve the protein expression level, solubility, and purity, such as expression vector, host bacteria, and various purification methods. Through renaturation and refolding, the immunogen displayed correct epitopes, which improved the probability of antibody recognition of the protein in its natural conformation.

Peptide antigen

For the most difficult-to-express antigens or those with low specificity, we choose the best region after a professional analysis, and synthesize a peptide (later coupled to KLH) as antigen to immunize the animals.

Our quality control procedures are very strict, ensuring that each synthetic peptide maintains high quality and high purity.

SPF animals


Pure SPF (Specific pathogen Free) class of experimental animals are used for immunization.

Variable immunization cycle


According to the needs of customer and the titer test results of post-fourth immunization anti-serum, the immunization cycle is adjusted appropriately.

Antigen affinity purification


Antibodies are purified by protein A and antigen affinity chromatography.
Cross-purification can be provided by ABclonal to obtain more specific antibodies when needed.

  • Anti-serum Provides approximately 50-80 ml of anti-serum per animal
  • Purification method

Antibody validation


Guaranteed results:

  • Affinity purified custom polyclonal antibody.
  • 1 ng of protein antigen or 100 ng of peptide antigen is detected when the antibody is diluted 1/1000.

ABclonal's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).We guarantee that a single antigen will be injected into both animals.

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5mL per rabbit)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (~25mL per rabbit)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (~50mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Note: After Day 35, the Scheduled days in this protocol are approximate. They depend on the titer test.

High-quality scientific research papers have been successfully published in well - known journals regarding ABclonal antibodies. We will continue to offer outstanding custom polyclonal antibody services with in an effort to service the scientific research community at large. Our goal is always to provide our customers with "the Better Antibody".

Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.
  4. Our customer service is available 24/7.