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Free antigen design for customized application-specific polyclonal antibody.
We provide customized services for producing application-specific polyclonal antibodies used in your research. Leverage our years of experiences in polyclonal antibody production: from antigen design, immunogen preparation, antibody development, antibody purification, modification, validation, to immunoassay development.
Free Antigen Design – Based on experience, data, and characteristics of your desired target. learn more
Step 1: Cross-examine the sequence provided by the customer with the information in multiple databases, such as NCBI.
Step 2: Screen suitable, immunogenic protein or peptide candidates.
Step 3: Compare BLAST searches with genome or protein databases to identify ideal protein or peptide candidates.
Step 4: Perform protein alignments of homologs/orthologs/isoforms.
Step 5: Refer to relevant publications to better understand the 3D structure of the protein and the protein-protein/protein-DNA interaction.
We immunize rabbits, mice, rats, and guinea pigs to generate custom polyclonal antibodies against peptide antigens and protein antigens. Both peptide and protein antigens can be provided by you or prepared by us.
ABclonal's standard protocol for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option and a popular choice among researchers.
The protocol uses two New Zealand White rabbits that are specific-pathogen-free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA). We guarantee that the same antigen will be injected into both animals.
|Control Serum Collection||Day 0||Pre-immune bleed (1 mL per rabbit)|
|Primary Injection||Day 1||Immunize with 0.50 mg of antigen in CFA, 10 SQ sites|
|1st Booster||Day 14||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|2nd Booster||Day 28||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 35||Bleed (-25 mL per rabbit)|
|3rd Booster||Day 42||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 56, 58||Two bleeds (total of -50 mL per rabbit)|
|ELISA Test||Day 60||ELISA titration (results available online) Verify disposition of rabbits Decide to continue or terminate|
|Purification||Optional||6-10 mg of purified antibodies|
High-quality scientific research papers have been successfully published in well-known journals regarding ABclonal antibodies.