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ABclonal also offers a number of optional services to complement our antibody production services.
Phase | Description | |
---|---|---|
Phase I | 2-3 weeks | Set up Gene synthesis – light chain and heavy chain provided by the customer |
Phase II | 2-3 weeks | Express & validate
|
Phase Ⅲ | 2-3 weeks | Expand and purify antibodies in 1 L of culture fluid Protein G or affinity purification (the yield is dependent on the nature of the antibody) |
Phase IV | 2 weeks | Select high-expression CHO cell line (optional) CHO cells >100 mg/L |
Freshly-harvested hybridoma cells
5 × 106 cells or more hybridoma cell lysate: entirely treated with TRIzol Reagent
RNA concentration no less than 100 ng/μl
RNA volume no less than 20 μl
RNA bands will be clear, with no significant degradation
Antibody isotyping identification
3-5 business days (alternatively, provided by customer)
12-16 business days
21-28 business days
Detailed performance report
Sequence data
Alignments of heavy and light chains
Plasmid containing V-region sequence
Note:
Please send us the samples as soon as possible using dry ice or liquid nitrogen. Delivery time will speed up significantly if customers provide the correct isotype and species information.