ABclonal also offers a number of optional services to complement our antibody production service.

Antibody Expression


Expression Mouse Monoclonal Antibody Package Includes
  • Gene synthesis light chain and heavy chain
  • Express and purify recombinant antibody
  • Validate monoclonal antibody by ELISA
  • Expand and purify antibody
Deliverables
  • Purified antibody from 1L
  • ELISA and other test reports


Phase Description
Phase I 2-3 weeks

Set up

Gene systhesis light chain and heavy chain provided by customer and subclone them into expression vector Subclone into ABclonal proprietary expression vector

Phase II 2-3 weeks

Express & validate

  • Small scale express antibody in CHO
  • Validate recombinant antibody by ELISA
  • Send sample to customer to validate
Phase II 2-3 weeks

Expand and purify antibodies in 1L

Protein G or Affinity Purification [The ylied is depeneded on the nature of the antibody]

Phase IV 2 weeks

Select high expression CHO cell line [Optional] CHO cells

>100mg/L

Antibody Sequencing Service


  • An antibody sequence is critical for monoclonal antibody engineering, antibody humanization, optimization of functionality, database cataloguing, and antibody-related drug development. ABclonal provides customers with none but the highest-quality hybridoma sequencing services. Hybridoma sequencing mainly involves the sequencing of monoclonal antibodies’ variable regions.
  • Highlights:
Sequence Accuracy
Quality control
guaranteeing 100%
accuracy
Cost-efficiency
Competitive pricing
for excellent service

Fast Turn-around Time
On average,
15-business-days

Broad Experience
Experience sequencing human, mouse, rat, and rabbit mAbs
  • Starting from a hybridoma cell line, we offer the following services

Complete Package Details


Samples
  • Freshly harvested hybridoma cells
    5 × 106 cells or more Hybridoma cell lysate: entirely treated with TRIZOL Reagent

Total RNA

RNA concentration no less than 100ng/μl
RNA volume no less than 20 μl
RNA bands will be clear, with no significant
degradation

Service Timeline
  • Antibody isotyping identification
    3-5 business days (alternatively, provided by customer)

Variable domain sequencing

12-16 business days

Full-length antibody sequencing

21-28 business days

Deliverables
  • Detailed performance report

  • Sequence data

  • Alignments of heavy and light chains

  • Plasmid containing V-region sequence

Note:
Please send the samples as rapidly as possible using dry ice or liquid nitrogen. Delivery time largely sped up if correct isotype & species information are provided by customers.

Other services


Peptide
Peptide synthesis and conjugation/Peptide synthesis without conjugation/Peptide conjugation
Ascites Production Service
Five mouse-scales of ascites production
Purication
Antigen affinity purification,protein A/G/L purification
Evaluation
ELISA/Western blot/Dot blot/IHC/IF/IP/Co-IP/ChIP-seq
Labeling
Biotin, FITC, 5-FAM, rhodamine, HRP,etc.
Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.
  4. Our customer service is available 24/7.