ABclonal also offers a number of optional services to complement our antibody production services.
Antibody Expression
Expression Mouse Monoclonal Antibody Package Includes
- Gene synthesis – light chain and heavy chain
- Express and purify recombinant antibody
- Validate monoclonal antibody using ELISA
- Expand and purify antibody
Deliverables
- Antibodies purified from 1 L of culture fluid
- ELISA and other test reports
| Phase |
|
Description |
| Phase I |
2-3 weeks |
Set up
Gene synthesis – light chain and heavy chain provided by the customer
Subclone into ABclonal proprietary expression vector |
| Phase II |
2-3 weeks |
Express & validate
- Small-scale xpress antibody in CHO
- Validate recombinant antibody using ELISA
- Send sample to the customer to validate
|
| Phase Ⅲ |
2-3 weeks |
Expand and purify antibodies in 1 L of culture fluid
Protein G or affinity purification (the yield is dependent on the nature of the antibody) |
| Phase IV |
2 weeks |
Select high-expression CHO cell line (optional) CHO cells
>100 mg/L |
Antibody Sequencing Service
- An antibody sequence is critical for monoclonal antibody engineering, antibody humanization, optimization of functionality, database cataloguing, and antibody-related drug development. ABclonal provides customers with none but the highest-quality hybridoma sequencing services. Hybridoma sequencing mainly involves the sequencing of monoclonal antibodies’ variable regions.
Sequence Accuracy
Quality control
guaranteeing 100%
accuracy
Cost-Efficient
Competitive pricing
for excellent service
Fast Turnaround Time
On average,
15 business days
Broad Experience
Experienced in sequencing human, mouse, rat, and rabbit mAbs
- Starting from a hybridoma cell line, we offer the following services
Complete Package Details
Total RNA
RNA concentration no less than 100 ng/μl
RNA volume no less than 20 μl
RNA bands will be clear, with no significant degradation
Variable domain sequencing
12-16 business days
Full-length antibody sequencing
21-28 business days
Deliverables
-
Detailed performance report
-
Sequence data
-
Alignments of heavy and light chains
-
Plasmid containing V-region sequence
Note:
Please send us the samples as soon as possible using dry ice or liquid nitrogen. Delivery time will speed up significantly if customers provide the correct isotype and species information.
Other services
- Peptide
- Peptide synthesis and conjugation / Peptide synthesis without conjugation / Peptide conjugation
- Ascites Production Service
- Five mouse-scales of ascites production
- Purication
- Antigen affinity purification, protein A / G / L purification
- Evaluation
- ELISA / Western Blot / Dot Blot / IHC / IF / IP / Co-IP / ChIP-seq
- Labeling
- Biotin, FITC, 5-FAM, rhodamine, HRP, etc.
