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StepWise DNA Lib Prep Kit (RK20202)

Datasheet

Catalog No.: RK20202
Sequencer: Illumina

The StepWise DNA Library Preparation Kit is designed to generate high-quality next generation sequencing (NGS) DNA libraries compatible with Illumina® platforms.

Product Description

The StepWise DNA Library Preparation Kit is designed to generate high-quality next generation sequencing (NGS) DNA libraries compatible with Illumina(R) platforms. Starting from fragmented input-DNA (500 pg - 1000 ng), the kit contains all the enzymes and buffers for end-repairing, dA-tailing, adapter ligation and library amplification. Due to the flexible protocol, the kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
Tube name8 RXN24 RXN96 RXN
End PreparationEnd Prep Buffer130 μl338 μl1.3 ml
End Prep Enzymes30 μl78 μl300 μl
dA-PrepdA-Prep Buffer490 μl1274 μl4.9 μl
dA-Prep Enzymes10 μl26 μl100 μl
Adapter LigationLigase MM255 μl663 μl2.55 mL
Ligase Mix20 μl52 μl200 μl
Amplification2X PCR Master Mix250 μl650 μl2.5 mL
10X PCR Primers40 μl130 μl500 μl
Low-EDTA TE1 ml3 ml10 ml
PEG/NaCl Solution1 ml3 ml10 ml
The Low-EDTA TE buffer and PEG/NaCl solution can be stored at room temperature or 4°C.
All other components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.
Mechanical or enzymatic methods for DNA fragmentation
Multiplex adapters (cat. no. RK20282, RK20283, RK20284, RK20285, RK20292, RK20293, RK20294, RK20295) compatible with Illumina(R) platforms
100% ethanol (ACS grade)
Nuclease-free water
PCR strip tubes or plates
Magnetic stand
Thermocycler
AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880)
Pipettes and multichannel pipettes
Aerosol resistant pipette tips
Microcentrifuge
Vortex mixer
Agilent Bioanalyzer or comparable method to assess the quality of DNA library

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