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Rapid DNA Lib Prep kit (RK20200)

Datasheet

Catalog No.: RK20200
Sequencer: Illumina

The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina platforms. The entire protocol can be completed in 2 hours.

Adavantages

Input DNA amount: 1ng-1ug
High DNA library conversion, and less PCR amplification cycles;
Suitable for FFPE DNA and cfDNA
Reduced PCR bias
Lower cost per library

Product Description

The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
Tube name8 RXN24 RXN96 RXN
End PreparationEnd Prep Buffer100 μl260 μl1000 μl
End Prep Enzymes30 μl78 μl300 μl
Adaptor LigationLigase MM165 μl429 μl1650 μl
Ligase Mix30 μl78 μl300 μl
Low-EDTA TE Buffer1000 μl2 mL5 mL
Amplification2X PCR Master Mix250 μl650 μl2.5 mL
10X PCR Primers40 μl120 μl500 μl
The Low-EDTA TE buffer can be stored at room temperature or 4°C.
All other components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.
Mechanical or enzymatic methods for DNA fragmentation
Multiplex adapters (cat. no. RK20282, RK20283, RK20284, RK20285, RK20292, RK20293, RK20294, RK20295) compatible with Illumina(R) platforms
100% ethanol (ACS grade)
Nuclease-free water
PCR strip tubes or plates
Magnetic stand
Thermocycler
AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880)
Pipettes and multichannel pipettes
Aerosol resistant pipette tips
Microcentrifuge
Vortex mixer
Agilent Bioanalyzer or comparable method to assess the quality of DNA library

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