| Cat. No. | Specification | Price | ||
|---|---|---|---|---|
| RK20302 | 24 RXN | $557 | Add to Cart | |
| RK20302 | 96 RXN | $1897 | Add to Cart | |
| Box | Box Name | Tube Name | 24 rxns | 96 rxns |
| Box 1 | Poly(A) mRNA Purification Module (RK20341) | Oligo d(T)25 Capture Beads | 480 μl | 1920 μl |
| mRNA Binding Buffer | 12 ml | 48 ml | ||
| Washing Buffer | 19.2 ml | 76.8 ml | ||
| Tris Buffer | 1.2 ml | 4.8 ml | ||
| Box 2 | mRNA-seq Lib Prep Module for Illumina (RK20350) | 2X Frag/Elute Buffer | 144 μl | 576 μl |
| RT Reagent | 192 μl | 768 μl | ||
| First Strand Synthesis Enzyme Mix | 48 μl | 192 μl | ||
| Second Strand Synthesis Reaction Buffer | 192 μl | 768 μl | ||
| Second Strand Synthesis Enzyme Mix | 96 μl | 384 μl | ||
| Nuclease-free Water | 1 ml X 2 | 8 ml | ||
| End Prep Buffer | 240 μl | 960 μl | ||
| End Prep Enzyme Mix | 72 μl | 288 μl | ||
| Ligation Buffer | 396 μl | 1584 μl | ||
| Ligase Mix | 72 μl | 288 μl | ||
| 2X PCR Mix | 600 μl | 2×1200 μl | ||
| Low-EDTA TE | 1 mL X 3 | 10 ml | ||
| Box 3 | RNA Adapter Module (24 Indices)(RK20345) | RNA Truncated Adapter | 60 μl | 240 μl |
| RNA Universal Primer | 60 μl | 240 μl | ||
| RNA Index Primer | 2.5 μl* | 10 μl* |
10 ng, 100ng or 1 μg of Total RNA was used as input for libraries after using Poly(A) mRNA Purification Module (ABclonal, Cat. RK20341), and libraries were then prepared using mRNA-seq Lib Prep kits from different manufacturers. ABclonal mRNA-seq Lib Prep Module for Illumina showed significantly higher efficiency of first cDNA synthesis (left panel) and library yield (right panel) than competitors.
Poly(A)-containing mRNA was isolated from 10 ng, 100ng or 1 μg of Total RNA, and libraries were prepared using the ABclonal mRNA-seq Lib Prep Kit. Libraries were sequenced on an Illumina platform. FPKM correlation analysis of the transcripts indicates excellent correlation of transcript expression between different total RNA inputs.
10 ng, 100ng or 1 μg of Total RNA was mixed into the same amount of ERCC RNA Spike-in Control Mixes(ambion, 4456740) and libraries were then prepared using the mRNA-seq Lib Prep kits from different manufacturers. Libraries were sequenced on an Illumina platform. ABclonal mRNA-seq Lib Prep Kit showed significantly higher ERCC transcript correlation than competitors.
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