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mRNA-seq Lib Prep Kit (RK20302)

Datasheet

Catalog No.: RK20302
Sequencer: Illumina

The ABclonal mRNA-seq Lib Prep Kit for IlluminaR comprises four processing modules: Poly(A) mRNA Purification, First Strand Synthesis, Second cDNA Synthesis and DNA Library Preparation.

Product Description

The ABclonal mRNA-seq Lib Prep Kit for IlluminaR comprises four processing modules: Poly(A) mRNA Purification, First Strand Synthesis, Second cDNA Synthesis and DNA Library Preparation. The kit includes all of the enzymes and buffers for poly(A) mRNA enrichment and mRNA-seq library construction, from 10 - 1000 ng of total RNA isolated from a wide variety of eukaryotic species
The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
BoxModule NameTube Color & Name24 Reactions (RK20302M)96 Reactions (RK20302L)
Box-1Poly(A) mRNA Purification Module (RK20341)Oligo d(T)25 Capture Beads480 μl1920 μl
mRNA Binding Buffer12 ml48 ml
Washing Buffer19.2 ml76.8 ml
Tris Buffer1.2 ml4.8 ml
Box-2First Strand Synthesis Module (RK20353)2X Frag/Elute Buffer144 μl576 μl
RT Reagent192 μl768 μl
First Strand Synthesis Enzyme Mix48 μl192 μl
Second Strand Synthesis Module (RK20346)Second Strand Synthesis Reaction Buffer192 μl768 μl
Second Strand Synthesis Enzyme Mix96 μl384 μl
Nuclease-free Water2 ml8 ml
DNA Lib Prep Module (RK20347)End Prep Buffer240 μl960 μl
End Prep Enzyme Mix72 μl288 μl
Ligation Buffer396 μl1584 μl
Ligase Mix72 μl288 μl
2X PCR Mix600 μl2×1200 μl
Low-EDTA TE2.5 ml10 ml
Box-3RNA Adapter Module (24 Indices)(RK20345)RNA Truncated Adapter60 μl240 μl
RNA Universal Primer60 μl240 μl
RNA Index Primer2.5 μl*10 μl*
Box-1: 2-8°C;
Do NOT freeze the Oligo (dT)25 Capture Beads.
Box-2: -20°C;
Box-3: -20°C.
100% ethanol (ACS grade)
Nuclease-free water
Nuclease-free PCR tubes or plates
Magnetic stand
Thermocycler
AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880)
Pipettes and multichannel pipettes
Aerosol resistant pipette tips
Microcentrifuge
Vortex mixer
Agilent Bioanalyzer or comparable method to assess the quality of stranded mRNA-seq library

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