Product Type > NGS Library Prep > RNA Library Prep > RNA Library Prep Kits

mRNA-seq Lib Prep Kit for Illumina (RK20302)

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Cat. No. Specification Price
RK20302 24 RXN $557
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RK20302 96 RXN $1897
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Description

The ABclonal mRNA-seq Lib Prep Kit for Illumina ® provides a method for generating non-directional (non-strand-specific) RNA library , which contains Poly(A) mRNA Purification Module(ABclonal, Cat. RK20341), mRNA-seq Lib Prep Module for Illumina®( ABclonal, Cat. RK20350) and RNA adaptors for Illumina. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified mRNA templates. The recommended total RNA input is 10 ng-1 μg.
Workflow Chart

Storage

Box-1: 2-8°C;
Box-2: -20°C;
Box-3: -20°C.

Product Information

Product Components

Box Box Name Tube Name 24 rxns 96 rxns
Box 1 Poly(A) mRNA Purification Module (RK20341) Oligo d(T)25 Capture Beads 480 μl 1920 μl
mRNA Binding Buffer 12 ml 48 ml
Washing Buffer 19.2 ml 76.8 ml
Tris Buffer 1.2 ml 4.8 ml
Box 2 mRNA-seq Lib Prep Module for Illumina (RK20350) 2X Frag/Elute Buffer 144 μl 576 μl
RT Reagent 192 μl 768 μl
First Strand Synthesis Enzyme Mix 48 μl 192 μl
Second Strand Synthesis Reaction Buffer 192 μl 768 μl
Second Strand Synthesis Enzyme Mix 96 μl 384 μl
Nuclease-free Water 1 ml X 2 8 ml
End Prep Buffer 240 μl 960 μl
End Prep Enzyme Mix 72 μl 288 μl
Ligation Buffer 396 μl 1584 μl
Ligase Mix 72 μl 288 μl
2X PCR Mix 600 μl 2×1200 μl
Low-EDTA TE 1 mL X 3 10 ml
Box 3 RNA Adapter Module (24 Indices)(RK20345) RNA Truncated Adapter 60 μl 240 μl
RNA Universal Primer 60 μl 240 μl
RNA Index Primer 2.5 μl* 10 μl*
Product Data

1.ABclonal mRNA-seq libraries showed high efficiency of first cDNA synthesis and library yield

10 ng, 100ng or 1 μg of Total RNA was used as input for libraries after using Poly(A) mRNA Purification Module (ABclonal, Cat. RK20341), and libraries were then prepared using  mRNA-seq Lib Prep kits from different manufacturers. ABclonal mRNA-seq Lib Prep Module for Illumina showed significantly higher efficiency of first cDNA synthesis (left panel) and library yield (right panel) than competitors.

2.ABclonal mRNA-seq libraries retain highly correlation of transcript expression between different total RNA inputs

Poly(A)-containing mRNA was isolated from 10 ng, 100ng or 1 μg of Total RNA, and libraries were prepared using the ABclonal mRNA-seq Lib Prep Kit. Libraries were sequenced on an Illumina platform. FPKM correlation analysis of the transcripts indicates excellent correlation of transcript expression between different total RNA inputs.

3.ABclonal mRNA-seq libraries retain high ECRR transcript expression correlation

10 ng, 100ng or 1 μg of Total RNA was mixed into the same amount of ERCC RNA Spike-in Control Mixes(ambion, 4456740) and libraries were then prepared using the mRNA-seq Lib Prep kits from different manufacturers. Libraries were sequenced on an Illumina platform. ABclonal mRNA-seq Lib Prep Kit showed significantly higher ERCC transcript correlation than competitors.

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