Product Type > NGS Library Prep > Adapters > Adapters for DNA/RNA Library Prep

Truncated DNA Adapter Kit for Illumina Set_C (48 indices) (RK20294)

Manual

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Description

ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D (48 indices) is ideally suited for sample preparation for next-generation sequencing on the illumina platform. Truncated DNA adapters have higher DNA ligation efficiency, lower or no adapter-dimers than full-length Y-shape DNA adapters. ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D box contains truncated adapter, universal PCR primer and 48 PCR index oligos. When using truncated DNA adapters, a minimum number of PCR amplification cycles (2-3) may be required to complete adapter sequences required for hybridization of the templates to the flowcell surface, and for annealing of sequencing primers. The kit can NOT be used to perform PCR-free DNA library preparation. ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D (48 indices) can be used in the ABclonal Rapid DNA Lib Prep Kit and ABclonal StepWise DNA Lib Prep Kit.
ZyCloning Assembly Workflow
NameIndexVolumeNameIndexvolumeTruncated Adapter-600μLUniversal PCR Primer-600μLPCR Index 1TTACCGAC12.5μLPCR Index 25TTCCAGGT12.5μLPCR Index 2AGTGACCT12.5μLPCR Index 26TCATCTCC12.5μLPCR Index 3TCGGATTC12.5μLPCR Index 27GAGAGTAC12.5μLPCR Index 4CAAGGTAC12.5μLPCR Index 28GTCGTTAC12.5μLPCR Index 5TCCTCATG12.5μLPCR Index 29GGAGGAAT12.5μLPCR Index 6GTCAGTCA12.5μLPCR Index 30AGGAACAC12.5μLPCR Index 7CGAATACG12.5μLPCR Index 31CAGTGCTT12.5μLPCR Index 8TCTAGGAG12.5μLPCR Index 32CTTGCTAG12.5μLPCR Index 9CGCAACTA12.5μLPCR Index 33TGGAAGCA12.5μLPCR Index 10CGTATCTC12.5μLPCR Index 34AGCTAAGC12.5μLPCR Index 11GTACACCT12.5μLPCR Index 35GAACGGTT12.5μLPCR Index 12CGGCATTA12.5μLPCR Index 36GGAATGTC12.5μLPCR Index 13TCGTCTGA12.5μLPCR Index 37TACGGTCT12.5μLPCR Index 14AGCCTATC12.5μLPCR Index 38CCAGTATC12.5μLPCR Index 15CTGTACCA12.5μLPCR Index 39TCTACGCA12.5μLPCR Index 16AGACCTTG12.5μLPCR Index 40GTAACCGA12.5μLPCR Index 17AGGATAGC12.5μLPCR Index 41GACGTCAT12.5μLPCR Index 18CCTTCCAT12.5μLPCR Index 42CTTACAGC12.5μLPCR Index 19GTCCTTGA12.5μLPCR Index 43TCCATTGC12.5μLPCR Index 20TGCGTAAC12.5μLPCR Index 44AGCGAGAT12.5μLPCR Index 21CACAGACT12.5μLPCR Index 45CAATAGCC12.5μLPCR Index 22TTACGTGC12.5μLPCR Index 46AAGACACC12.5μLPCR Index 23CCAAGGTT12.5μLPCR Index 47CCAGTTGA12.5μLPCR Index 24CACGCAAT12.5μLPCR Index 48TGGTGAAG12.5μL" data-lightbox="imgs" target="_blank">
Product Features
  • Truncated adapter，high ligation efficiency and less adapter dimer；
  • PCR free is not supported, and the library needs at least 2-3 rounds of amplification;
  • 1 universal i5 primers and 48 indexed i7 primers;
  • Flexible combination with ABclonal library preparation kits.
Storage
Stored at -20°C. The shelf life of all reagents is one year when stored properly.

Product Information

Product Components
ComponentsSize 1 (48 RXN)Size 2 (96 RXN)Size 3 (240 RXN)
Truncated Adapter120240600
Universal PCR Primer120240600
PCR index 1-48 Primer2.5 μl5 μl12.5 μl

Documents

Manual

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