Description
The ABclonal Small RNA Lib Prep Kit for Illumina V2® include all reagents in 3' Adapter Ligation, 5' Adapter Ligation, First Strand cDNA Synthesis, Library Enrichment and PAGE Gel Elution. The obtained target library is sequenced on the Illumina sequencing platform. For standard miRNA libraries, the fragment size is about 150 bp. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified small RNA templates. The recommended total RNA input is 100 ng-1 μg.
During small RNA library preparation, the ABclonal Small RNA Lib Prep Kit for Illumina V2® can be used with one of the Small RNA Adapter Module 96 Index for Illumina kits(ABclonal, Cat. RK20357/RK20358/RK20359/RK20360) for library enrichment and index labeling of each sample library.
Workflow Chart
Storage
5' Adapter: -80°C
DNA PAGE Gel Elution Buffer: room temperature
Other components: -20°C.
Small RNA Adapter Module Package: -20°C.
Product Components
Module Name | Tube Name | 24 rxns | 96 rxns |
3' Adapter Ligation | 3' Adapter | 24 μL | 96 μl |
2X 3' Adapter Ligation Buffer | 240 ul | 960 ul |
3' Adapter Ligation Enzymes II | 48 ul | 192 ul |
5' Adapter Ligation | 5' Adapter | 24 μL | 96 μl |
5' Adapter Ligation Buffer | 24 μL | 96 μl |
5' Adapter Ligation Enzymes | 24 μL | 96 μl |
First Strand cDNA Synthesis | RT Primer | 24 μl | 96 μl |
5X First Strand Synthesis Buffer | 192 μl | 768 μl |
First Strand Synthesis Enzymes | 48 μl | 192 μl |
Library Enrichment | 2X LA PCR Mix | 1.2 ml | 4.8 ml |
Nuclease-free Water | 1 ml | 2 ml |
PAGE Gel Elution | Quick Load pBR322-Mspl Digest DNA Marker | 240 μl | 960 μl |
6X Loading Buffer | 120 μl | 480 μl |
DNA PAGE Gel Elution Buffer | 6 μl | 24 μl |
Linear Acrylamide | 24 μl | 96 μl |
Documents
Certificate of Compliance
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
Inquire About This Product
Submit your question about RK20307 below and we will get back to you
within one business day.
Alternatively, call us at 888.754.5670.