Product Type > NGS Library Prep > RNA Library Prep > RNA Library Prep Kits

Small RNA Lib Prep Kit for Illumina V2 (RK20307)

COA

Discontinued

Description

The ABclonal Small RNA Lib Prep Kit for Illumina V2® include all reagents in 3' Adapter Ligation, 5' Adapter Ligation, First Strand cDNA Synthesis, Library Enrichment and PAGE Gel Elution. The obtained target library is sequenced on the Illumina sequencing platform. For standard miRNA libraries, the fragment size is about 150 bp. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified small RNA templates. The recommended total RNA input is 100 ng-1 μg.
During small RNA library preparation, the ABclonal Small RNA Lib Prep Kit for Illumina V2® can be used with one of the Small RNA Adapter Module 96 Index for Illumina kits(ABclonal, Cat. RK20357/RK20358/RK20359/RK20360) for library enrichment and index labeling of each sample library.
Workflow Chart

Storage

5' Adapter: -80°C
DNA PAGE Gel Elution Buffer: room temperature
Other components: -20°C.
Small RNA Adapter Module Package: -20°C.

Product Information

Product Components

Module Name Tube Name 24 rxns 96 rxns
3' Adapter Ligation 3' Adapter 24 μL 96 μl
2X 3' Adapter Ligation Buffer 240 ul 960 ul
3' Adapter Ligation Enzymes II 48 ul 192 ul
5' Adapter Ligation 5' Adapter 24 μL 96 μl
5' Adapter Ligation Buffer 24 μL 96 μl
5' Adapter Ligation Enzymes 24 μL 96 μl
First Strand cDNA Synthesis RT Primer 24 μl 96 μl
5X First Strand Synthesis Buffer 192 μl 768 μl
First Strand Synthesis Enzymes 48 μl 192 μl
Library Enrichment 2X LA PCR Mix 1.2 ml 4.8 ml
Nuclease-free Water 1 ml 2 ml
PAGE Gel Elution Quick Load pBR322-Mspl Digest DNA Marker 240 μl 960 μl
6X Loading Buffer 120 μl 480 μl
DNA PAGE Gel Elution Buffer 6 μl 24 μl
Linear Acrylamide 24 μl 96 μl

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Inquire About This Product


Submit your question about RK20307 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Products Categories
Request a free sample
Contact us to order