Products > NGS Lib Prep Kits > Rapid Plus DNA Lib Prep kit

Rapid Plus DNA Lib Prep kit (RK20208)

Datasheet

Catalog No.: RK20208
Sequencer: Illumina

The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours.

Product Description

The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.
The following workflow illustrates the processes of Rapid DNA Lib Prep Kit.

Tube name8 RXN24 RXN96 RXN
End PreparationEnd Prep Buffer70 μl168 μl700 μl
End Prep Enzymes30 μl78 μl300 μl
Damaged DNA Repair Enzymes30 μl78 μl300 μl
Adaptor LigationLigation Buffer300 μl780 μl3 ml
Ligation Enzymes100 μl240 μl1000 μl
Amplification2X PCR Master Mix250 μl650 μl2.5 mL
10X PCR Primers50 μl130 μl500 μl
All components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.
Mechanical or enzymatic methods for DNA fragmentation
Multiplex adapters (cat. no. RK20282, RK20283, RK20284, RK20285, RK20292, RK20293, RK20294, RK20295) compatible with Illumina® platforms
100% ethanol (ACS grade)
Nuclease-free water
PCR strip tubes or plates
Magnetic stand
Thermocycler
AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880)
Pipettes and multichannel pipettes
Aerosol resistant pipette tips
Microcentrifuge
Vortex mixer
Agilent Bioanalyzer or comparable method to assess the quality of DNA library

Inquire About This Product

Submit your question about RK20208 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Request a free sample
Contact us to order