The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
Input DNA amount: 1ng-1ug High DNA library conversion, and less PCR amplification cycles; Suitable for FFPE DNA and cfDNA Reduced PCR bias Lower cost per library
The Low-EDTA TE buffer can be stored at room temperature or 4°C. All other components should be stored at -20°C. The shelf lives of all reagents are one year when stored properly.
End Prep Buffer
End Prep Enzymes
Low-EDTA TE Buffer
2X PCR Master Mix
10X PCR Primers
Publishing research using RK20200? Please let us know so that we can cite the reference in this datasheet.
Somatic variants in new candidate genes identified in focal cortical dysplasia type II