Product Type > NGS Library Prep > DNA Library Prep > DNA Library Prep Kits

Rapid DNA Lib Prep Kit for Illumina (RK20200)

Publications (6)



The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
Product Features

  • Input DNA amount: 1ng-1ug
    High DNA library conversion, and less PCR amplification cycles;
    Suitable for FFPE DNA and cfDNA
    Reduced PCR bias
    Lower cost per library
Workflow Chart


The Low-EDTA TE buffer can be stored at room temperature or 4°C.
All other components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.

Product Information

Product Components

Tube name 8 RXN 24 RXN 96 RXN
End Preparation End Prep Buffer 100 μl 260 μl 1000 μl
End Prep Enzymes 30 μl 78 μl 300 μl
Adaptor Ligation Ligase MM 165 μl 429 μl 1650 μl
Ligase Mix 30 μl 78 μl 300 μl
Low-EDTA TE Buffer 1000 μl 2 mL 5 mL
Amplification 2X PCR Master Mix 250 μl 650 μl 2.5 mL
10X PCR Primers 40 μl 120 μl 500 μl



Publishing research using RK20200? Please let us know so that we can cite the reference in this datasheet.

Inquire About This Product

Submit your question about RK20200 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Products Categories
Request a free sample
Contact us to order