Product Type > NGS Library Prep > DNA Library Prep > DNA Library Prep Kits

Rapid CE DNA Lib Prep Kit for Illumina V2 (RK20254)

Datasheet COA

Discontinued

Description

The Rapid CE DNA Lib Prep kit for Illumina V2 provides a streamlined and efficient method for the construction of DNA libraries for next generation sequencing (NGS) on Illumina® platforms. This kit is an updated version of the original Rapid CE DNA Lib Prep kit (Cat. Nos. RK20212) that has been further optimized to provide improved PCR amplification efficiency. The entire protocol can be completed within 2 hours. Starting from fragmented input DNA (1 - 1000 ng), the kit contains all necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for the construction of a PCR-free library from either 100 ng of high-quality fragmented genomic DNA or 10 ng circulating cell-free DNA (cfDNA). Optional PCR primers are provided in the kit for users who prefer self-selected high-fidelity DNA polymerases for library amplification.
Product Features

  • Easy to operate and the entire protocol can be completed in 2.5 hours;
  • Applicable to common genomes, cfDNA/ctDNA, FFPE DNA, ChIP DNA, PCR products, cDNA products, etc;
  • Wide range of applications for whole genome sequencing, exon and target sequencing, ChIP seq, amplicon sequencing;
  • Strict quality control including component pollution test, functional test verification, application scenario test and stability test between product batches.
Workflow Chart

Storage

All components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.

Product Information

Product Components

Tube name 8 RXN 24 RXN 96 RXN
End Preparation End Prep Buffer 80 μL 240 μL 960 μL
End Prep Enzymes 24 μL 72 μL 288 μL
Adaptor Ligation Ligase MM 132 μL 396 μL 1584 μL
Ligase Mix 24 μL 72 μL 288 μL
Amplification Gloria Nova HS 2X PCR Mix for NGS 200 μL 600 μL 2400 μL
10X PCR Primers 40 μL 120 μL 480 μL
Product Data

Documents

Certificate of Compliance

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Lot number

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