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FS Pro DNA Lib Prep Kit for Illumina (RK20261)

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Cat. No. Specification Price
RK20261 96 RXN $2100
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Description

The FS Pro DNA Lib Prep Kit for Illumina is designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit integrates DNA fragmentation with end repair and dA tailing into one step, and it can be directly followed by adapter ligation without purification. With greatly simplified experimental flow, the entire experiment can be completed within 2 hours. This kit is compatible with DNA samples from different species and different origins. Optimization of reagents and protocol greatly reduced rates of false positives, significantly improving sequencing yield and overall results, including with FFPE DNA samples. This kit is suitable for the construction of PCR-Free libraries (e.g., 100 ng of high-quality genomic DNA).
Product Features

  • Perform fragmentation, end repair and dA-tailing with a single enzyme mix;
  • Entire experiment completed within 2 hours.;
  • Generate a wide range of insert sizes using vary fragmention time;
  • Fewer inversions and lower chimeras;
Workflow Chart

Storage

All components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.

Product Information

Product Components

Components 8 RXN 24 RXN 96 RXN
Fragmentation, End Repair & dA Tailing FS Pro Buffer I 40 μL 120 μL 480 μL
FS Pro Enzymes I 80 μL 240 μL 960 μL
1X TE Buffer 280 μL 840 μL 1.68 mL
Adapter Ligation FS Pro Ligation Buffer I 160 μL 480 μL 1.92 mL
Ligation Enzymes 40 μL 240 μL 960 μL
Amplification Gloria Nova HS 2X PCR Mix for NGS 200 μL 600 μL 2.4 mL
10X PCR Primers 40 μL 120 μL 480 μL
Product Data

1. Generate a wide range of insert sizes using vary fragmention time.

100 ng human blood gDNA initial template was treated with this kit. Fragmentation was performed 32°C for 5/10/15/20 /25/30 min.

2. Lower false positives

100 ng FFPE DNA was starting template for library construction using this kit and other kits. Compared with other kits, FS Pro DNA Lib Prep Kit for Illumina has fewer inversions and lower chimeras. This indicates that the library construction using FS kit has less impact on subsequent analysis, and the results are more realistic.

Documents

Certificate of Compliance

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Publications

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