Product Type > NGS Library Prep > Adapters > Adapters for DNA/RNA Library Prep

Dual DNA Adapter 96 Kit Extended for Illumina (RK20296)

Datasheet COA

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Description

Dual DNA Adapter 96 Kit for Illumina contains adapters and combinatorial dual index (CDI) primers for multiplex sample preparation for Next Generation Sequencing on Illumina sequencing platforms. The kit provides universal truncated adapters at 15 μM as well as 8 unique i5 Primers and 12 unique i7 Primers 20 μM. The i5 Primers and i7 Primers can be combined to generate up to 96 different primer combinations for barcoding samples. The index length for the CDI primers is 8 nucleotides.
Tube covers for i5 Primers and i7 Primers differ in color for ease of identification. Ensure each combination is correct prior to use.
Product Features

  • Truncated adapter,high ligation efficiency and less adapter dimer;
  • PCR free is not supported, and the library needs at least 2-3 rounds of amplification;
  • 8 i5 primers, 12 i7 primers, 96 indexes in total (8 X 12);
  • Flexible combination with ABclonal library preparation kits.
ZyCloning Assembly Workflow

Components 96 RXN
Truncated Adapter 480 μL
i509-i516 Primer 30 μL each
i713-i724 Primer 20 μL each
Storage

All components should be stored at -20~C.
The shelf lives of all reagents are one year when stored properly.

Product Information

Product Components

Component Color 8 RXN 24 RXN 96 RXN 240 RXN
Truncated Adapter yellow 40 μL 120 μL 480 μL 1200 μL
i501 Primer blue 10 μL 15 μL 30 μL 75 μL
i502 Primer blue 10 μL 15 μL 30 μL 75 μL
i503 Primer blue 15 μL 30 μL 75 μL
i504 Primer blue 15 μL 30 μL 75 μL
i505 Primer blue 30 μL 75 μL
i506 Primer blue 30 μL 75 μL
i507 Primer blue 30 μL 75 μL
i508 Primer blue 30 μL 75 μL
i701 Primer purple 5 μL 10 μL 20 μL 50 μL
i702 Primer purple 5 μL 10 μL 20 μL 50 μL
i703 Primer purple 5 μL 10 μL 20 μL 50 μL
i704 Primer purple 5 μL 10 μL 20 μL 50 μL
i705 Primer purple 10 μL 20 μL 50 μL
i706 Primer purple 10 μL 20 μL 50 μL
i707 Primer purple 20 μL 50 μL
i708 Primer purple 20 μL 50 μL
i709 Primer purple 20 μL 50 μL
i710 Primer purple 20 μL 50 μL
i711 Primer purple 20 μL 50 μL
i712 Primer purple 20 μL 50 μL

Documents

Certificate of Compliance

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Lot number

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