Product Type > NGS Library Prep > RNA Library Prep > RNA Library Prep Modules

First Strand Synthesis Module (RK20353)

Publications (5) Datasheet

Select size and quantity
Cat. No. Specification Price
RK20353 24 RXN $158
Add to Cart
RK20353 96 RXN $528
Add to Cart

Description

ABclonal First Strand Synthesis Module (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
Storage

Store at -20°C.

Product Information

Product Components

Components24 RXN(RK20353M)96 RXN(RK20353L)500 RXN(RK20353XL)
2×Frag/Elute Buffer132μL528 μL1.25 mL ×2
RT Reagent192μL768 μL4 mL
First Strand Synthesis Enzyme Mix48μL192 μL1 mL

Documents

Publications

Publishing research using RK20353? Please let us know so that we can cite the reference in this datasheet.

Inquire About This Product


Submit your question about RK20353 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Products Categories
Request a free sample
Contact us to order