Product Type > NGS Library Prep > RNA Library Prep > RNA Library Prep Kits

First Strand Synthesis Module (Stranded) (RK20342)

Discontinued

Description

ABclonal First Strand Synthesis Module (Stranded) (RK20342) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Strand Specificity Reagent in this module contains Actinomycin D to improve strand specificity and can be used with ABclonal Second Strand Synthesis Module (Stranded) (RK20343) to make strand-specific RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
Storage

Store at -20°C.

Product Information

Product Components

Components24 RXN(RK20342M)96 RXN(RK20342L)500 RXN(RK20342XL)
2×Frag/Elute Buffer132μL528 μL1.25 mL ×2
RT Strand Specificity Reagent192μL768 μL4 mL
First Strand Synthesis Enzyme Mix48μL192 μL1 mL

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