We have created custom mouse monoclonal antibodies for numerous applications including functional assays, immunoprecipitation, IHC, IF, flow cytometry, Western blot, and ELISA.


  • Peptide antigen design and preparation

    Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity of >80% peptide conjugation to KLH

  • Protein antigen design

    Recombinant protein preparation

  • Customer-supplied immunogen (protein or peptide)
  • Immunization of mice (5 animals)
  • One primary subcutaneous injection of 100 ug conjugated peptide/protein in CFA
  • Five subcutaneous injections of 50 ug conjugated peptide/protein in IFA
  • Serum titration
  • Hybridoma fusion (best animal)
  • Clone screening
  • Stabilization of 2-5 positive clones
  • 500 mL cultures of one clone
  • Protein G purification

Note: If there are no specific requests, 0.02% sodium merthiolate will be added to the antisera and antibody products.

Express Immunization

ABclonal provides rapid immunizations within 2-5 weeks. This is suitable for the preparation of mouse monoclonal antibodies and polyclonal antibodies. Compared with conventional adjuvants, we have found our protocols to have a faster turnaround and to use less antigens.

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.


Mouse monoclonal antibodies
  • Hybridoma development packages and monoclonal antibody production services
Guaranteed results
  • Positive WB clone
  • Other applications (optional and limited guarantee available)
  • You choose (based on our screening result and your own validation) which immunized mouse to fuse and which cell lines to subclone and expand
One-stop service
  • We are a one-stop service capable of preparing the immunogen through antibody sequencing
Fast and cost-effective screening
  • Advanced antibody detection platform
  • Top engineer of antibody validation
High-quality antigens
  • Free antigen design
  • Suitable recombinant protein expression
  • High-purity peptides run in HPLC and MS

Immunization Schedule

ABclonal's standard option for mouse monoclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses five mice that are Specific-Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA). We guarantee that the same antigen will be injected into all five animals.

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (50-100 µl per mouse)
Primary Injection Day 1 Immunize with 0.50 mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Test-bleed (50~100 µl per mouse)
3rd Booster Day 42 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56 Send bleeds to customer for validation (50~100 µl per mouse)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Pre-fusion booster Day 62 Boost with 0.25 mg of antigen in saline, intraperitoneal
Fusion and ELISA screening Day 76 Fuse myeloma cells and spleen cells, screen clones
ELISA and WB screening Day 83 Screen clones
Subcloning and ELISA test Day 97 Subclone to ensure monoclonal lines and ELISA test
Expansion and WB test Day 104 Expansion clones, then freeze stock;
Send supernatants to customer for validation
Delivery Day 110 Deliver the product customer choose and final report
Note: After Day 35, the scheduled days in this protocol are approximations and are dependent on the titer test.
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.