• ABclonal is a leader in the Research and Development of custom mouse monoclonal antibody service. Our team strives to deliver successful monoclonal antibody production to customers every time. We work with each customer to develop a solution for both the simplest and most complicated mouse monoclonal development.
  • We have created custom mouse monoclonal antibodies for numerous applications including functional assays, immunoprecipitation, IHC, IF, flow cytometry, Western blot, and ELISA.
  • After doing thousands of projects a year, we have found that the most crucial step toward getting a helpful mouse mAb can be found in the very beginning. The production team works hard to develop a high-quality antigen that will create a strong immunogenic response.

Package


  • Peptide antigen design and preparation

    Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 80%eptide conjugation to KLH

  • Protein antigen design

    Recombinant protein preparation

  • Customer supplied immunogen(protein or peptide)
  • Immunization of mice (5 animals)
  • One primary subcutaneous injection of 100 ug conjugated peptide/protein in CFA
  • Five subcutaneous injections of 50 ug conjugated peptide/protein in IFA
  • Serum titratio
  • Hybridoma fusion (best animal)
  • Clone screening
  • Stabilization of 2-5 positive clones
  • 500ml cultures of 1 clones
  • Protein G purification

Note

If there is no specific request, 0.02% sodium merthiolate will be added to the antisera and antibody products.

Express Immunization


ABclonal provides rapid immunizations within 2-5 weeks. This is suitable for the preparation of mouse monoclonal antibodies and polyclonal antibodies. Compared with conventional adjuvants, we have found our protocols to have a faster turnaround and to use less antigen.

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.

Highlights


Mouse monoclonal antibodies
  • Hybridoma development packages and monoclonal antibody production services
Guarantee result
  • Positive WB clone
  • Other applications (optional and limited guarantee available)
Customizable
  • You choose (based on our screening result and your own validation) which immunized mouse to fuse and which cell lines to subclone and expand
One-stop service
  • We are a one-stop service capable of preparing the immunogen through antibody sequencing
Fast and cost-effective screening
  • Advanced antibody detection platform
  • Top engineer of antibody validation
High-quality antigen
  • Free antigen design
  • Suitable recombinant protein expression
  • High purity peptides run in HLPC and MS

High Throughput Screening


ABclonal can provide most validations in- house and we can improve the screening rate of positive cell lines with excellent antibody detection experts and advanced high- throughput antibody detection platform.

Optional screening


ABclonal's standard option for mouse monoclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses five mice that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA). We guarantee that a single antigen will be injected into five animals.

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (50-100ul per mouse)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Test-bleed (50~100uL per mouse)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56 Send bleeds to customer for validation (50~100uL per mouse)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Pre-fusion booster Day 62 Boost with 0.25mg of antigen in saline, intraperitoneal
Fusion and ELISA screening Day 76 Fuse myeloma cells and spleen cells, screen clones
ELISA and WB screening Day 83 Screen clones
Subcloning and ELISA test Day 97 Subclone to ensure monoclonal lines and ELISA test
Expansion and WB test Day 104 Expansion clones, then freeze stock;
Send supernatants to customer for validation
Delivery Day 110 Deliver the product customer choose and final report
Note: After Day 35, the Scheduled days in this protocol are approximate. They depend on the titer test.
Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.
  4. Our customer service is available 24/7.