Custom Mouse Monoclonal Antibody Production

Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.

Our custom mouse monoclonal antibody service adopts the hybridoma (fusion) technology to produce large numbers of highly specific monoclonal antibodies.

Antibody-secreting B cells are immortalized by merging B lymphocytes and myeloma cells in the immunized mouse. The hybridoma cell line secreting the monoclonal antibody is then selected using limited dilution cloning. The monoclonal antibody is produced on a large scale by amplifying the culture of the cell strain.

Highlights

  • Double Verification – Our procedure focuses on testing the mouse bleeds by WB (endogenous lysates) besides ELISA for mouse selection and sub cloning screening step.
  • Guaranteed Results – For WB antibody

  • Antigen Option – Antigens can be provided by you or prepared by us.

Mouse Monoclonal Antibody Production Process

Deliverables

1-3 selected hybridoma cell lines

100μg purified antibody from each selected cell line

Free cell banking of selected cell lines at ABclonal for 6 months

0.1-2mg antigen if provided by ABclonal (0.1mg protein antigen or 2mg peptide antigen)

QA/QC report of the antigen if provided by ABclonal

Project report

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