Custom Mouse Monoclonal Antibody Production

Monoclonal antibodies (mAbs) have a much higher specificity and lower between-batch variability compared to pAbs.

Our custom mouse monoclonal antibody service adopts the hybridoma (fusion) technology to produce large numbers of highly specific monoclonal antibodies.

Antibody-secreting B cells are immortalized by merging B lymphocytes and myeloma cells in the immunized mouse. The hybridoma cell line secreting the monoclonal antibody is then selected using limited dilution cloning. The monoclonal antibody is produced on a large scale by amplifying the culture of the cell strain.

Highlights

  • Double Verification – Our procedure focuses on testing the mouse bleeds by WB (endogenous lysates) besides ELISA for mouse selection and sub cloning screening step.
  • Guaranteed Results – For WB antibody

  • Antigen Option – Antigens can be provided by you or prepared by us.

Mouse Monoclonal Antibody Production Process

Deliverables

5mg of purified antibody from best hybridoma line

Up to two of the best hybridoma cell lines

QA/QC report for the antigen if provided by ABclonal

1-2mg antigen if provided by ABclonal

Project Report

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