Mouse monoclonal antibodies are still widely used in research and therapeutic applications as a cost-effective, multi-purpose discovery tool. While ABclonal also offers the SMabTM technology for antibody development, our optimized hybridoma pipeline is a reliable service for high quality customized antibody production suited for your project goals.

After immunization, antibody-secreting B cells are immortalized by fusion to myeloma cell lines, and the resultant hybridomas are screened using a client-directed customizable protocol to select for the desired antigen-specific antibody. ABclonal then transfers the hybridoma to our large-scale antibody production team to deliver the final reagent to accommodate your breakthrough research needs.

Our Mouse Monoclonal Service Provides

One-stop service – from consultation, antigen design and synthesis to antibody purification and validation.

Flexible optimization at each stage.

FREE cell banking at ABclonal for 6 months.

Claims no Intellectual Property or royalties for the resulting antibody product.


Hands On Expertise
Our dedicated project management and scientific teams work with you every step of the way to:

  • Customize protocols to meet your specific project goals
  • Provide updates to keep projects on track
  • Timely adjustments based on your feedback

Flexibility at Every Step
Your customized workflow can be modified to suit your unique requirements:

  • Antigen design leveraging our years of expertise combined with artificial intelligence (AI)-assisted algorithms, or provide your own antigen
  • 21-day rapid immunization schedule available
  • Repeat certain phases where desired
  • Select additional clones

Be The Next Success Story

Virus-neutralizing Antibody

ABclonal generated mouse hybridomas against the universal epitope for multiple human enteroviruses. Several of the hybridomas recognized different human enteroviruses, out of which two (10G2 and 1F2) presented virus-neutralizing activity. The neutralization potency of the purified ascites is greater than 1:2048 as tested using ELISA. Both hybridomas recognize conformational epitopes that have important biological activities in the virus, and a third hybridoma recognized the universal epitope for multiple human enteroviruses.

Diagnostic ELISA Pair Development

We successfully developed a mouse hybridoma-rabbit polyclonal ELISA pair against MRGPRX2, an important companion diagnostic target for drug-induced allergies. It mediates pseudo-allergic reactions characterized by histamine release, inflammation, and airway contraction.

Mouse Monoclonal Antibody Production Process



  • Antigen designn
  • Antigen synthesis and expression

9-10 WEEKS


  • Conventional or accelerated immunization protocol
  • Titer testing


Fusion and

  • Fusion clone screening by indirect ELISA
  • Addition screening assays available upon request
  • Up to 40 positive supernatants upon request (200 mL/sup)


Subcloning and

  • Subclone screening
  • Small-scale antibody purification
  • Isotype identification
  • Additional screening and characterization assays available upon request
  • Up to 10 purified mAb (100 mg/clone)


Cell Expansion &
Antibody Production

  • Antibody purification
  • Antibody validation by indirect ELISA
  • Additional validation, scale-up, and antibody engineering available upon request


  • 100ug purified mAb x (up to) 10 clones
  • Hybridoma Cell Line x 1
  • 1mg purified mAb x (up to) 1 clone
  • Final Project Report