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Mouse monoclonal antibodies are still widely used in research and therapeutic applications as a cost-effective, multi-purpose discovery tool. While ABclonal also offers the SMabTM technology for antibody development, our optimized hybridoma pipeline is a reliable service for high quality customized antibody production suited for your project goals.
After immunization, antibody-secreting B cells are immortalized by fusion to myeloma cell lines, and the resultant hybridomas are screened using a client-directed customizable protocol to select for the desired antigen-specific antibody. ABclonal then transfers the hybridoma to our large-scale antibody production team to deliver the final reagent to accommodate your breakthrough research needs.
One-stop service – from consultation, antigen design and synthesis to antibody purification and validation.
Flexible optimization at each stage.
FREE cell banking at ABclonal for 6 months.
Claims no Intellectual Property or royalties for the resulting antibody product.
Hands On Expertise
Our dedicated project management and scientific teams work with you every step of the way to:
Flexibility at Every Step
Your customized workflow can be modified to suit your unique requirements:
ABclonal generated mouse hybridomas against the universal epitope for multiple human enteroviruses. Several of the hybridomas recognized different human enteroviruses, out of which two (10G2 and 1F2) presented virus-neutralizing activity. The neutralization potency of the purified ascites is greater than 1:2048 as tested using ELISA. Both hybridomas recognize conformational epitopes that have important biological activities in the virus, and a third hybridoma recognized the universal epitope for multiple human enteroviruses.
We successfully developed a mouse hybridoma-rabbit polyclonal ELISA pair against MRGPRX2, an important companion diagnostic target for drug-induced allergies. It mediates pseudo-allergic reactions characterized by histamine release, inflammation, and airway contraction.
4-6 WEEKS
Antigen
Preparation
9-10 WEEKS
Immunization
4-5 WEEKS
Fusion and
Screening
6-7 WEEKS
Subcloning and
Screening
5-6 WEEKS
Cell Expansion &
Antibody Production
Deliverables:
100ug purified mAb x (up to) 10 clonesHybridoma Cell Line x 11mg purified mAb x (up to) 1 cloneFinal Project Report
