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phi29 DNA Polymerase (RK21002)

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Cat. No. Concentration Specification Price
RK2100210, 000 U/ml250 U$35
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RK2100210, 000 U/ml1250 U$140
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Description

phi29 DNA Polymerase is a replicative polymerase from the Bacillus subtilis phage phi29. phi29 DNA Polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3'→5' proofreading exonuclease activity. phi29 DNA Polymerase has advantaged in replication requiring a high degree of strand displacement and/or processive synthesis, as well as high fidelity replication at moderate temperatures.
Product Components

Components Cat. No.
phi29 DNA Polymerase (10, 000 U/ml)RM20501
10X phi29 DNA Polymerase Reaction BufferRM20113
Product Information

Featured Specification
ApplicationsIsothermal Amplification
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Storage Conditions10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Twee-20, 0.5% (v/v) NP-40, 50% Glycerol, pH 7.5 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X phi29 DNA Polymerase Reaction Buffer, Incubate at 30°C.
Reaction Buffer or Mix1X phi29 DNA Polymerase Reaction Buffer:
50 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM MgCl2, 4 mM DTT, pH7.5 @ 25°C.
Heat Inactivation65°C for 10 min
5' - 3' ExonucleaseNo
3' - 5' ExonucleaseYes
Strand Displacement+
Protein M.W.Theoretical 67000 daltons
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