Products > Molecular Biology > Restriction Endonucleases

XbaI (RK21112)

Datasheet

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Cat. No. Concentration Specification Price
RK2111220, 000 U/mL3000 U$55
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RK2111220, 000 U/mL15000 U$230
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Description

5'…T/CTAGA...3'
3'…AGATC/T...5'
Product Components

Components Cat. No.
XbaI (20, 000 U/ml)RM21614
10X Buffer CutSRM20103
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-/HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µL.
Storage Conditions10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutS, Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation65°C for 20 min
Methylation Sensitivity
dam methylationBlocked by Overlapping
dcm methylationNot Sensitive
CpG MethylationNot Sensitive
Activity in Abclonal Buffers
CutACutBCutCCutS
10%100%75%100%
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