Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling.
|Taq Antibody (5, 000 U/mL)||RM20325|
|Applications||Multiplex PCR, Specialty PCR, PCR , Routine PCR|
|Unit Definition||One unit is defined as the amount of antibody required to inhibit greater than 90% one unit of Taq DNA polymerase after a 10 minute incubation at 55°C.|
|Storage Conditions||10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA630, 50% Glycerol, pH7.4 @ 25°C|
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