Description
Taq Antibody is a mouse monoclonal antibody that specifically binds to Taq DNA polymerase. When this antibody is bound to Taq, the enzyme is rendered inactive. It provides an antibody-mediated hot start that enhances the specificity and sensitivity of PCR. Inhibition is completely reversed when the temperature is raised during the first denaturing step of thermal cycling.
Product Features
- Good blocking, and inhibits non-specific amplification effectively.
- Does not affect Taq amplification efficiency, antibodies are inactivated during PCR pre-denaturation, and does not affect normal PCR reactions.
- Rigorous quality control, DNase/RNase free, no exonuclease/endonuclease activity, and no microbial genomic residues by PCR detection.
Applications
Multiplex PCR, Specialty PCR, PCR , Routine PCR
Storage
-20°C
Product Components
Components | 100 U | 500 U |
Taq Antibody (5, 000 U/mL) | 20 μL | 100 μL |
Product Data
Blocking efficiency test
- Different amounts of DNA were added to the reaction system as the templates, then set up a group called "Non-Hot Start" without Taq antibody and another group called "Hot Start" with Taq antibody for amplification. The results showed: “non-Hot Start” group produced more unknown components; “Hot Start”group added with ABclonal Taq enzyme antibody had no obvious non-specific amplification and had better blocking specific activity than competing products.
Documents
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