Product Type > Molecular Biology Reagents > Ligase > RNA Ligases

T4 RNA Ligase 2, truncated (RK20505)

Datasheet COA

Select size and quantity
Cat. No. Concentration Specification Price
RK20505 200, 000 U/ml 10000 U $170
Add to Cart

Description

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2 truncated is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. Unlike the full length ligase, T4 Rnl2 truncated is unable to adenylate the 5´ end of the substrate, and as a result it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA. This enzyme, also known as Rnl2 (1-249) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.
Applications

RNA ligase
Storage

-20°C

Product Information

Product Components

Components2, 000 U10, 000 U
T4 RNA Ligase 2, truncated (200, 000 U/mL)10 μL50 μL
10X T4 RNA Ligase Reaction Buffer500 μL500 μL
50% PEG 8000500 μL1.25 mL

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Inquire About This Product


Submit your question about RK20505 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Products Categories
Contact us to order