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T4 DNA Polymerase (5, 000 U/ml) (RK20539)

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Cat. No. Concentration Specification Price
RK205395, 000 U/ml200 U$56
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RK205395, 000 U/ml1000 U$224
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Description

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function.
It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
Product Components

Components Cat. No.
T4 DNA Polymerase (5, 000 U/ml)RM21302
10X ABuffer BRM20126
Product Information

Featured Specification
Product SourcePurified from a strain of E. coli that carries the T4 DNA Polymerase gene.
ApplicationsBlunting, Polymerases for DNA Manipulation, PCR
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Storage Conditions100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X ABuffer B, Incubate at 12°C
Reaction Buffer or Mix1X ABuffer B:
10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 @ 25°C
Heat Inactivation75°C for 20 min
5' - 3' ExonucleaseNo
3' - 5' ExonucleaseYes
Strand DisplacementNo
Activity in Abclonal Buffers
ABufferAABufferBABufferCABufferS
60%100%100%100%
Protein M.W.Theoretical 104000 daltons
Error Rate~ 1x10-6 bases
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