Product Type > Molecular Biology Reagents > Nucleic Acid Modifying Enzymes > DNA Manipulation

T4 DNA Polymerase (5, 000 U/mL) (RK20539)

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Cat. No. Concentration Specification Price
RK20539 5, 000 U/ml 200 U $56
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RK20539 5, 000 U/ml 1000 U $224
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Description

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function.
It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
Applications

Blunting, Polymerases for DNA Manipulation, PCR
Storage

-20°C

Product Information

Product Components

ComponentsConcentration200 U1, 000 U
T4 DNA Polymerase (5, 000 U/ml)5, 000 U/mL40 μL200 μL
10X ABuffer B10X1.25 mL1.25 mL X 2

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