T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function.
It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
|T4 DNA Polymerase (5, 000 U/ml)||RM21302|
|10X ABuffer B||RM20126|
|Product Source||Purified from a strain of E. coli that carries the T4 DNA Polymerase gene.|
|Applications||Blunting, Polymerases for DNA Manipulation, PCR|
|Unit Definition||One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.|
|Storage Conditions||100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C|
|Reaction Conditions||1X ABuffer B, Incubate at 12°C|
|Reaction Buffer or Mix||1X ABuffer B: |
10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 @ 25°C
|Heat Inactivation||75°C for 20 min|
|5' - 3' Exonuclease||No|
|3' - 5' Exonuclease||Yes|
|Activity in Abclonal Buffers|
|Protein M.W.||Theoretical 104000 daltons|
|Error Rate||~ 1x10-6 bases|
Inquire About This Product
Submit your question about RK20539 below and we will get back to you within one business day.