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T4 DNA Ligase (RK21501)

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Cat. No. Concentration Specification Price
RK21501400, 000 U/ml16000 U$38
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RK21501400, 000 U/ml80000 U$168
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Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
Product Components

Components Cat. No.
T4 DNA LigaseRM21501
10X T4 DNA ligase Reaction BufferRM20108
Product Information

Featured Specification
Product SourceAn E.coli strain that carries the T4 DNA ligase gene.
ApplicationsCloning Ligation
Unit DefinitionOne unit is defined as the amount of enzyme required to ligate 50% ligase of HindIII digestion fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl over 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.
Storage Conditions10 mM Tris-HCl,50 mM KCl,1 mM DTT,0.1 mM EDTA,50% Glycerol,pH7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X T4 DNA Ligase Reaction Buffer.
Reaction Buffer or Mix1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl,10 mM MgCl2,10 mM DTT,1 mM ATP,pH7.5 @ 25°
Heat Inactivation65°C for 10 min.
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