Products > Molecular Biology > Restriction Endonucleases

StuI (RK21162)

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Cat. No. Concentration Specification Price
RK2116210, 000 U/ml1000 U$45
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RK2116210, 000 U/ml5000 U$183
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Description

5'…AGG/CCT...3'
3'…TCC/GGA...5'
Product Components

Components Cat. No.
StuI (20, 000 U/ml)RM21662
10X Buffer CutURM20102
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutU. Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutU:
20 mM Tris-acetate,10 mM Magnesium acetate,50 mM Potassium acetate,100 µg/ml BSA,pH 7.9 @25℃
Heat Inactivation80°C for 20 min
Methylation Sensitivity
dam MethylationNot Sensitive
dcm MethylationBlocked by Overlapping
CpG MethylationNot Sensitive
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