Products > Molecular Biology > Restriction Endonucleases

SpeI (RK21113)

Datasheet

Discontinued

Description

5'…A/CTAGT...3'
3'…TGATC/A...5'
Product Components

Components Cat. No.
SpeI (10, 000 U/mL)RM21615
10X Buffer CutSRM20103
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg pXba-XbaI DNA in 1 hour at 37°C in a total reaction volume of 50 µL.
Storage Conditions10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/mL BSA, 0.15% Triton X-100, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutS. Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation80°C for 20 min
Methylation Sensitivity
dam methylationNot Sensitive
dcm methylationNot Sensitive
CpG MethylationNot Sensitive
Activity in Abclonal Buffers
CutACutBCutCCutS
75%100%25%100%
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