| Cat. No. | Concentration | Specification | Price | ||
|---|---|---|---|---|---|
| RK21116 | 20, 000 U/mL | 2000 U | $40 | Add to Cart | |
| RK21116 | 20, 000 U/mL | 10000 U | $160 | Add to Cart | |
| Components | Cat. No. |
|---|---|
| SmaI (20, 000 U/ml) | RM21618 |
| 10X Buffer CutS | RM20103 |
| Featured | Specification | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||||
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 25°C in a total reaction volume of 50 µL. | ||||||||
| Storage Conditions | 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||||
| Storage Temperature | -20°C | ||||||||
| Reaction Conditions | 1X Buffer CutS, Incubate at 25°C . | ||||||||
| Reaction Buffer or Mix | 1X Buffer CutS: 50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C | ||||||||
| Heat Inactivation | 65°C for 20 min | ||||||||
| Methylation Sensitivity |
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| Activity in Abclonal Buffers |
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