Products > Molecular Biology > Restriction Endonucleases

SmaI (RK21116)

Datasheet

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Cat. No. Concentration Specification Price
RK2111620, 000 U/mL2000 U$40
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RK2111620, 000 U/mL10000 U$160
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Description

5'…CCC/GGG...3'
3'…GGG/CCC...3'
Product Components

Components Cat. No.
SmaI (20, 000 U/ml)RM21618
10X Buffer CutSRM20103
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 25°C in a total reaction volume of 50 µL.
Storage Conditions10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutS, Incubate at 25°C .
Reaction Buffer or Mix1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation65°C for 20 min
Methylation Sensitivity
dam methylationNot Sensitive
dcm methylationNot Sensitive
CpG MethylationBlocked
Activity in Abclonal Buffers
CutACutBCutCCutS
10%10%10%100%
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