Products > Molecular Biology > RNA & DNA Modifying Enzymes > RNases

RNase H (RK20535)



Catalog No.: RK20535

Concentration: 5, 000 U/ml


RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

Product Component

RNase H (5, 000 U/ml)RM21305
10X RNase H Reaction BufferRM20142

Product information

Product SourceAn E.coli strain that carries the cloned RNase H gene (rnh) from E. coli.
ApplicationsRT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Unit DefinitionOne unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 ul in 20 minutes at 37°C.
Storage Conditions10 mM Tris-HCl, 50 mM KCl,0.1 mM EDTA, 1 mM DTT,200 μg/ml BSA,50% Glycerol,pH7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X RNase H Reaction Buffer, Incubate at 37°C
Reaction Buffer or Mix1X RNase H Reaction Buffer:
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C
Heat Inactivation65°C for 20 min

Inquire About This Product

Submit your question about RK20535 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order