Catalog No.: RK20535
Concentration: 5, 000 U/ml
|RNase H (5, 000 U/ml)||RM21305|
|10X RNase H Reaction Buffer||RM20142|
|Product Source||An E.coli strain that carries the cloned RNase H gene (rnh) from E. coli.|
|Applications||RT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR|
|Unit Definition||One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 ul in 20 minutes at 37°C.|
|Storage Conditions||10 mM Tris-HCl, 50 mM KCl，0.1 mM EDTA, 1 mM DTT，200 μg/ml BSA，50% Glycerol，pH7.4 @ 25°C|
|Reaction Conditions||1X RNase H Reaction Buffer, Incubate at 37°C|
|Reaction Buffer or Mix||1X RNase H Reaction Buffer: |
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C
|Heat Inactivation||65°C for 20 min|
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