Products > Molecular Biology > RNA & DNA Modifying Enzymes > RNases

RNase H (RK20535)

Datasheet

Summary

Catalog No.: RK20535

Concentration: 5, 000 U/ml

Background

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

Product Component

RNase H (5, 000 U/ml)RM21305
10X RNase H Reaction BufferRM20142

Product information

Product SourceAn E.coli strain that carries the cloned RNase H gene (rnh) from E. coli.
ApplicationsRT-PCR & cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Unit DefinitionOne unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 ul in 20 minutes at 37°C.
Storage Conditions10 mM Tris-HCl, 50 mM KCl,0.1 mM EDTA, 1 mM DTT,200 μg/ml BSA,50% Glycerol,pH7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X RNase H Reaction Buffer, Incubate at 37°C
Reaction Buffer or Mix1X RNase H Reaction Buffer:
50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C
Heat Inactivation65°C for 20 min

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