Products > Molecular Biology > Restriction Endonucleases

NheI (RK21111)

Datasheet

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Cat. No. Concentration Specification Price
RK2111110, 000 U/mL1000 U$50
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RK2111110, 000 U/mL5000 U$220
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Description

5'…G/CTAGC...3'
3'…CGATC/G...5'
Product Components

Components Cat. No.
NheI (10, 000 U/ml)RM21613
10X Buffer CutBRM20105
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 0.15% Triton X-100, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutB, Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutB:
50 mM NaCl, 10 mM Tris-HC, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9 @ 25°C.
Heat Inactivation65°C for 20 min
Methylation Sensitivity
dam methylationNot Sensitive
dcm methylationNot Sensitive
CpG MethylationBlocked by Some Combinations
of Overlapping
Activity in Abclonal Buffers
CutACutBCutCCutS
100%100%10%100%
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