Products > Molecular Biology > Restriction Endonucleases

MluI (RK21122)

Datasheet

Discontinued

Description

5'…A/CGCGT...3'
3'…TGCGC/A...5'
Product Components

Components Cat. No.
MluI (10, 000 U/mL)RM21623
10X Buffer CutCRM20106
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µL.
Storage Conditions10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutC. Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutC:
100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/mL BSA, pH 7.9 @ 25°C
Heat Inactivation80°C for 20 min.
Methylation Sensitivity
dam methylationNot Sensitive
dcm methylationNot Sensitive
CpG MethylationBlocked
Activity in Abclonal Buffers
CutACutBCutCCutS
10%50%100%25%
Documents

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