Products > Molecular Biology > PCR, qPCR & Amplification Technologies > LongerAmp Taq DNA Polymerase

LongerAmp Taq DNA Polymerase (RK20680)

Datasheet

ABclonal:LongerAmp Taq DNA Polymerase
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Cat. No. Concentration Specification Price
RK206801000 U$120
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RK206802500 U$258
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Description

LongerAmp Taq DNA Polymerase is a unique blend of Taq klenow and Deep Ocean DNA Polymerases. The 3´→5´ exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. LongerAmp Taq DNA Polymerase offers two fold higher fidelity than Taq klenow DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from human genomic DNA.
Product Components

Components Cat. No.
LongerAmp Taq DNA Polymerase (2, 500 U/ml)RM20312
5X LongerAmp Taq Reaction BufferRM20140
Product Information

Featured Specification
ApplicationsLong Range PCR; Colony PCR
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Storage Conditions10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA-630, 50% Glycerol, pH7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X LongerAmp Taq Reaction Buffer
Reaction Buffer or Mix1X LongerAmp Taq Reaction Buffer:
60 mM Tris-SO4, 20 mM (NH4)2SO4, 2 mM MgSO4, 3% Glycerol, 0.06% IGEPAL CA-630, 0.05% Tween 20, pH9.1 @ 25°C
Heat InactivationNo
5' - 3' ExonucleaseNo
3' - 5' ExonucleaseYes
Strand Displacement+
Resulting EndsMix of Blunt and Single-base 3´ Overhangs
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