Products > Molecular Biology > Restriction Endonucleases

KpnI (RK21104)


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Cat. No. Concentration Specification Price
RK2110410, 000 U/mL4000 U$42
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Product Components

Components Cat. No.
KpnI (10, 000 U/ml)RM21606
10X Buffer CutARM20104
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutA, Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutA:
10 mM Bis-Tris-Propane-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7 @ 25°C
Heat InactivationNo
Methylation Sensitivity
dam MethylationNot Sensitive
dcm MethylationNot Sensitive
CpG MethylationNot Sensitive
Activity in Abclonal Buffers

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