Gloria Nova HS DNA polymerase is an ideal enzyme for high fidelity PCR with excellent processivity. It is a novel engineered enzyme with comparable performance to Pyrococcus furiosus DNA polymerase.
With unique structure Gloria Nova HS DNA polymerase contains a recombinant synthesis enhancement domain to increase fidelity and extension speed. The antibody-mediated hot-start feature significantly inhibits non-specific amplifications at room temperature.
Gloria Nova is one of the thermostable DNA polymerases with strong 3’-5’ exonuclease activity (proofreading activity), which results in its extreme high fidelity, 10-15 times higher than Taq DNA polymerase and 6 times higher than Pyrococcus furiosus DNA polymerase.
The Gloria Nova HS PCR Kit is supplied with a 5X Gloria Nova HF Buffer and a 2.5X Gloria Nova GC Buffer. The 5X Gloria Nova HF Buffer is an optimized buffer for general high fidelity amplifications while the 2.5X Gloria Nova GC Buffer is used in the amplifications of problematic or GC-rich templates.
|Gloria Nova HS DNA Polymerase (2, 000 U/mL)||RM20405|
|5X Gloria Nova HF Buffer||RM20182|
|2.5X Gloria Nova GC Buffer||RM20183|
|dNTPs (10mM each)||RM20120|
|Applications||Long Range PCR, High-Fidelity PCR, Fast PCR , Multiplex PCR , Specialty PCR , PCR , Routine PCR|
|Storage Temperature||Upon receipt, store all components at -20°C.|
|5' - 3' Exonuclease||No|
|3' - 5' Exonuclease||Yes|
|Resulting Ends||Blunt end|
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