Product Type > Molecular Biology Reagents > qPCR > Dye-based qPCR

Genious 2X SYBR Green Fast qPCR Mix (RK21204)

Publications (56) Datasheet COA

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Cat. No. Concentration Specification Price
RK21204 2X 5 mL $170
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RK21204 2X 25 mL $730
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Description

Real-time Quantitative PCR (qPCR) is a powerful technique in detecting initial DNA input in a PCR reaction by fluorescence signal accumulation. ABclonal Genious 2X SYBR Green Fast qPCR Mix is a fluorescence reagent for qPCR reactions using the SYBR® Green I. This product provides real-time data on DNA amplification during PCR by performing quantitative fluorescence signal detection on SYBR® / FAM channels. This product uses the antibody-based hot-start Taq enzyme for amplification, which greatly improves the specificity of the product while ensuring the amplification effect. At the same time, by optimizing the qPCR Mix Buffer system, the product is suitable for multiple species, providing a powerful tool for multidisciplinary experimental needs.
This product is a 2X pre-mix enzyme, contains all the components required for qPCR except primers and templates, providing great convenience for experimental manipulation.
Product Features

  • SYBR Green I fluorescence combined with double-stranded DNA has high sensitivity and SNR.
  • Compatible with fluorescence quantitative PCR instruments requiring high concentration of ROX;
  • Hot-start Taq DNA polymerase was used to improve specificity.
  • Premixed reagent is easy to operate;
Applications

qPCR
Storage

﹣20 ℃ for long term storage and 4 ℃ for a short period and Light protection is demanded.

Product Information

Product Components

ComponentsSize
5 mL
Size
25 mL
Genious 2X SYBR Green Fast qPCR Mix (No ROX)1 mL X 51 mL X 25
50X ROX Dye I220 μL220 μL X 5
50X ROX Dye II220 μL220 μL X 5
Product Data

  • Mouse gDNA was used as a template for 14 gradient dilutions in 2-fold gradients using ABclonal Genious 2X SYBR Green Fast qPCR Mix
    Detection of the target gene GAPDH, the experimental results showed that with the increase of dilution, the CT value of the target gene showed regular changes, showing the excellent sensitivity of the qPCR reagent.

Documents

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Publications

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