Products > Molecular Biology > Restriction Endonucleases

EcoRV (RK21106)

Datasheet

ABclonal:EcoRV
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Cat. No. Concentration Specification Price
RK2110620, 000 U/mL4000 U$45
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Description

5'...GAT/ATC...3'
3'...CTA/TAG...5'
Product Components

Components Cat. No.
EcoRV (20, 000 U/ml)RM21604
10X Buffer CutSRM20103
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA,200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutS, Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation80°C for 20 min
Methylation Sensitivity
dam MethylationNot Sensitive
dcm MethylationNot Sensitive
CpG MethylationImpaired by Some Combinations of Overlapping
Activity in Abclonal Buffers
CutACutBCutCCutS
10%50%100%100%
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