Product Type > Molecular Biology Reagents > Ligase > DNA Ligases

E.coli DNA Ligase (RK20501)

Datasheet

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Cat. No. Concentration Specification Price
RK20501 10, 000 U/ml 200 U $38
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RK20501 10, 000 U/ml 1000 U $150
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Description

E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4°C - 37°C).
Applications

Non-Cloning Ligation
Storage

-20°C

Product Information

Product Components

Components200 U1, 000 U
E.coli DNA Ligase (10, 000 U/mL)20 μL100 μL
10X E.coli DNA Ligase Reaction Buffer50 μL250 μL

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