Products > Molecular Biology > Restriction Endonucleases

DpnI (RK21109)

Publication (1) Datasheet

ABclonal:DpnI
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Cat. No. Concentration Specification Price
RK2110920, 000 U/mL1000 U$50
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Description

5'...GmA/TC...3'
3'...CT/mAG...3'
Product Components

Components Cat. No.
DpnI (20, 000 U/ml)RM21610
10X Buffer CutSRM20103
Product Information

Featured Specification
ApplicationsRestriction Enzyme Digestion
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/ml BSA, 50% Glycerol pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X Buffer CutS, Incubate at 37°C.
Reaction Buffer or Mix1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation80°C for 20 min
Methylation Sensitivity
dam methylationNot Sensitive
dcm methylationNot Sensitive
CpG MethylationBlocked by Overlapping
Activity in Abclonal Buffers
CutACutBCutCCutS
100%100%75%100%
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