Products > Molecular Biology > RNA & DNA Modifying Enzymes > DNA Manipulation

DNA Polymerase I (E. coli) (RK20530)

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Cat. No. Concentration Specification Price
RK2053010, 000 U/ml500 U$43
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RK2053010, 000 U/ml2500 U$170
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Description

DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3´→ 5´ and 5´→ 3´ exonuclease activities. The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
It is applicable to nick translation of DNA for obtaining probes with a high specific activity and for second strand synthesis of cDNA.
Product Components

Components Cat. No.
DNA Polymerase I (E. coli) (10, 000 U/ml)RM20518
10X ABuffer BRM20126
Product Information

Featured Specification
Product SourceAn E.coli strain that carries an overexpressed copy of the polA gene.
ApplicationsPolymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, PCR
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Storage Conditions25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C
Storage Temperature-20°C
Reaction Conditions1X ABuffer B, Incubate at 37°C
Reaction Buffer or Mix1X ABuffer B:
10 mM Tris-HC, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, pH7.9 @ 25°C
Heat Inactivation75°C for 20 min
5' - 3' ExonucleaseYes
3' - 5' ExonucleaseYes
Strand DisplacementNo
Protein M.W.Theoretical 103000 daltons
Error Rate< 9x10-6 bases
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