Catalog No.: RK20537
Concentration: 5, 000 U/ml
|Antarctic Phosphatase (5, 000 U/ml)||RM21308|
|10X Antarctic Phosphatase Reaction Buffer||RM20152|
|Product Source||An E.coli strain that carries the T4 DNA ligase gene.|
|Applications||Dephosphorylation, RNA Modification, In vitro Synthesis (IVT)|
|Unit Definition||One unit is defined as the amount of enzyme that will dephosphorylate 1 ug of pUC19 vector DNA cut with a restriction enzyme generating 5' recessed ends in 30 minutes at 37°C. Dephosphorylation is defined as > 95% inhibition of recircularization in a self-ligation reaction and is measured by transformation into E. coli.|
|Storage Conditions||10 mM Tris-HCl, 1 mM MgCl2, 50% Glycerol, 0.01 mM ZnCl2, pH 7.4 @ 25°C|
|Reaction Conditions||1X Antarctic Phosphatase Reaction Buffer, Incubate at 37°C|
|Reaction Buffer or Mix||1X Antarctic Phosphatase Reaction Buffer: |
50 mM Bis-Tris-Propane-HCl, 1 mM MgCl2, 0.1 mM ZnCl2, pH 6 @ 25°C
|Heat Inactivation||80°C for 2 min|
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