Product Type > Molecular Biology Reagents > Reverse Transcription > Multifunctional Reverse Transcription Kit

ABScript II cDNA First-Strand Synthesis Kit (RK20400)

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RK20400 100 RXN $298
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Description

ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV, recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity, and all RNA, including mRNA, rRNA, and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.
Product Features

  • The low activity of RNase H is beneficial to the synthesis of long fragment cDNA, which can synthesize cDNA up to 10 KB.
  • The optimum temperature of the enzyme was 42℃ and the enzyme still has high activity at 48℃.
  • The specific cDNA yield was higher than that of ordinary M-MLV reverse transcriptase (37℃).
  • Suitable for coronavirus detection;
  • With a variety of RT primers, both mRNA and ncRNA can be used as reverse transcription templates.
Applications

cDNA first-strand Synthesis
Storage

-20°C

Product Information

Product Components

Components50 RXN100 RXN
ABScript II Enzyme Mix (10X)100 µL200 µL
ABScript II Reaction Mix (2X)600 µL1.25 mL
Oligo d(T)23VN (50 μM)100 µL200 µL
Random Primer Mix (60 μM)100 µL200 µL
dNTPs (10 mM each)50 µL100 µL
Nuclease-free H2O1.25 mL1.25 mL
ABScript II Enzyme Mix (10X)100 µL200 µL
Product Data

  • Using 1μg of HeLa cell total RNA as template, reverse transcription was performed with ABScript II cDNA First-Strand Synthesis Kit, and 1μL of cDNA was used as template to obtain clear and specific electrophoresis bands for genes of different lengths

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