ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.
Product Features
RT, qPCR one-tube reaction, and operation steps are reduced;
Compatible with multiple probe systems (at least quadruple probe);
Suitable for SARS-CoV-2 and various RNA virus detection;
Good stability, and the activity will not be significantly affected if stored at 37℃ for a week.
Applications
One Step probe RT-qPCR,RNA viruses detection
Storage
Upon receipt, store all components at -20°C.
Product Information
Product Components
Components
50 RXN (20 μL / RXN)
250 RXN (20 μL / RXN)
2X One Step RT-qPCR Probe Buffer IV
500 μL
1.25 mL X 2
One Step Probe Enzyme Mix IV
100 μL
500 μL
50X ROX Dye I
20 μL
100 μL
50X ROX Dye II
20 μL
100 μL
Nuclease-free ddH2O
500 μL
1.25 mL X 2
Product Data
1. SARS-CoV-2 detection
log
-2
-3
-4
-5
-6
-7
-8
E(%)
R2
FAM-ORF1ab
23.434114
26.416568
29.913333
33.676513
37.25661
40.837371
Undetermined
92.25
0.999
VIC-N
21.887704
24.968435
28.426713
32.089382
36.056823
40.761581
Undetermined
84.75
0.9949
Using the coronavirus sample as a template, and performed with 10-fold dilution. The results showed that product RK20412 can detect the ORF1 gene and N gene of the SARS-CoV-2 well.