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ABScript III One Step RT-qPCR Probe Kit with UDG V5 (RK20412)

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Cat. No.ConcentrationSpecificationPrice
RK204122X50 RXN$79
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RK204122X250 RXN$320
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Description

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.
Product Features

  • RT, qPCR one-tube reaction, and operation steps are reduced;
  • Contains dUTP/UDG anti-contamination system, prevents aerosol contamination effectively;
  • Compatible with multiple probe systems (at least quadruple probe);
  • Suitable for SARS-CoV-2 and various RNA virus detection;
  • Good stability, and the activity will not be significantly affected if stored at 37℃ for a week.
Applications

One Step probe RT-qPCR,RNA viruses detection
Storage

Upon receipt, store all components at -20°C.

Product Information

Product Components

Components50 RXN (20 μL / RXN)250 RXN (20 μL / RXN)
2X One Step RT-qPCR Probe Buffer IV500 μL1.25 mL X 2
One Step Probe Enzyme Mix IV100 μL500 μL
50X ROX Dye I20 μL100 μL
50X ROX Dye II20 μL100 μL
Nuclease-free ddH2O500 μL1.25 mL X 2
Product Data

1. SARS-CoV-2 detection
log -2 -3 -4 -5 -6 -7 -8 E(%) R2
FAM-ORF1ab 23.434114 26.416568 29.913333 33.676513 37.25661 40.837371 Undetermined 92.25 0.999
VIC-N 21.887704 24.968435 28.426713 32.089382 36.056823 40.761581 Undetermined 84.75 0.9949
  • Using the coronavirus sample as a template, and performed with 10-fold dilution. The results showed that product RK20412 can detect the ORF1 gene and N gene of the SARS-CoV-2 well.

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