Product Type > Molecular Biology Reagents > qPCR > One Step RT-qPCR

ABScript III One Step RT-qPCR Probe Kit with UDG V5 (RK20412)

Publication (1) Datasheet COA

Select size and quantity
Cat. No. Concentration Specification Price
RK20412 2X 50 RXN $79
Add to Cart
RK20412 2X 250 RXN $320
Add to Cart


ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.
Product Features

  • RT, qPCR one-tube reaction, and operation steps are reduced;
  • Contains dUTP/UDG anti-contamination system, prevents aerosol contamination effectively;
  • Compatible with multiple probe systems (at least quadruple probe);
  • Suitable for SARS-CoV-2 and various RNA virus detection;
  • Good stability, and the activity will not be significantly affected if stored at 37℃ for a week.

One Step probe RT-qPCR,RNA viruses detection

Upon receipt, store all components at -20°C.

Product Information

Product Components

Components50 RXN (20 μL / RXN)250 RXN (20 μL / RXN)
2X One Step RT-qPCR Probe Buffer IV500 μL1.25 mL X 2
One Step Probe Enzyme Mix IV100 μL500 μL
50X ROX Dye I20 μL100 μL
50X ROX Dye II20 μL100 μL
Nuclease-free ddH2O500 μL1.25 mL X 2
Product Data

1. SARS-CoV-2 detection
log -2 -3 -4 -5 -6 -7 -8 E(%) R2
FAM-ORF1ab 23.434114 26.416568 29.913333 33.676513 37.25661 40.837371 Undetermined 92.25 0.999
VIC-N 21.887704 24.968435 28.426713 32.089382 36.056823 40.761581 Undetermined 84.75 0.9949
  • Using the coronavirus sample as a template, and performed with 10-fold dilution. The results showed that product RK20412 can detect the ORF1 gene and N gene of the SARS-CoV-2 well.


Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number


Publishing research using RK20412? Please let us know so that we can cite the reference in this datasheet.

Inquire About This Product

Submit your question about RK20412 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Products Categories
Contact us to order