Description
ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.
Applications
PCR, RT-qPCR, RT-PCR and cDNA Synthesis, DNA Amplification, PCR & qPCR
Storage
-20°C
Product Components
Components | 20 RXN (50 μL/ RXN) | 100 RXN (50 μL / RXN) |
2X One Step RT-qPCR Probe Buffer | 500 μL | 1.25 mL X 2 |
One Step Probe HS Taq | 20 μL | 100 μL |
ABScript II RT Enzyme Mix | 20 μL | 100 μL |
50X ROX Dye I | 20 μL | 100 μL |
50X ROX Dye II | 20 μL | 100 μL |
Nuclease-free H2O | 500 μL | 1.25 mL X 2 |
Documents
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