Description
ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.
Product Components
Components
|
Cat. No.
|
---|
2X One Step RT-qPCR Probe Buffer | RM21462 |
One Step Probe HS Taq | RM21463 |
ABScript II RT Enzyme Mix | RM21464 |
50X ROX Dye I | RM21465 |
50X ROX Dye II | RM21466 |
RNase-free ddH2O | RM20214 |
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