ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.
|2X One Step RT-qPCR Probe Buffer||RM21462|
|One Step Probe HS Taq||RM21463|
|ABScript II RT Enzyme Mix||RM21464|
|50X ROX Dye I||RM21465|
|50X ROX Dye II||RM21466|
|Applications||PCR, RT-qPCR, RT-PCR and cDNA Synthesis, DNA Amplification, PCR & qPCR|
Inquire About This Product
Submit your question about RK20407 below and we will get back to you within one business day.