Products > Molecular Biology > PCR, qPCR & Amplification Technologies > cDNA Synthesis & Reverse Transcriptases

ABScript II One Step RT-qPCR Probe Kit (RK20407)

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Cat. No.SpecificationPrice
RK2040720 RXN$42
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RK20407100 RXN$168
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Description

ABScript II One Step RT-qPCR Probe Kit a ready-to-use kit allowing reverse transcription and subsequent probe-based qPCR in a single tube. It contains all components for RT-qPCR except primers, probes and RNA templates. The one-step format significantly improves sensitivity and effectively prevent contamination. The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription to a wide range of RNA template amount. After reverse transcription, the Hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. The ABScript II One Step RT-qPCR Probe Kit is an ideal product for high-speed analyses of low input RNA sample.
Applications

PCR, RT-qPCR, RT-PCR and cDNA Synthesis, DNA Amplification, PCR & qPCR
Storage

-20°C

Product Information

Product Components

Components20 RXN
(50 μL/ RXN)
100 RXN
(50 μL / RXN)
2X One Step RT-qPCR Probe Buffer500 μL1.25 mL X 2
One Step Probe HS Taq20 μL100 μL
ABScript II RT Enzyme Mix20 μL100 μL
50X ROX Dye I20 μL100 μL
50X ROX Dye II20 μL100 μL
Nuclease-free H2O500 μL1.25 mL X 2

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