Description
Real-time Quantitative PCR (qPCR) is a powerful technique in detecting initial DNA input in a PCR reaction by fluorescence signal accumulation. The DNA double strand bonded dye, SYBR® Green I is the most commonly used dye in qPCR. ABclonal 2X Universal SYBR Green Fast qPCR Mix contains de novel designed universal reference dye, which can realize higher signal resolution and suits for all currently used qPCR instruments (including high ROX mode, low ROX mode and No ROX mode required machines). It also contains Hot-Start Taq DNA polymerase to avoid unexpected amplification results.
Besides, ABclonal 2X Universal SYBR Green Fast qPCR Mix is an optimized qPCR reaction mix, It contains all required components in qPCR except primers and template. It is convenient for experiment and suitable for multiple species. The above features make it as an ideal experiment tool for gene quantitative research.
Product Features
- SYBR Green I fluorescence combined with double-stranded DNA has high sensitivity and SNR.
- Compatible with a variety of fluorescence quantitative PCR instruments;
- Hot-start Taq DNA polymerase was used to improve specificity.
Applications
qPCR
Storage
﹣20 ℃ for long term storage and 4 ℃ for a short period and Light protection is demanded.
Product Components
| Components | Size
500 RXN | Size
2, 500 RXN |
| 2X Universal SYBR Green Fast qPCR Mix | 1 mL X 5 | 1 mL X 25 |
Product Data
1. Consistent performance when using different instruments and modes
- Amplification plot of E. coli gDNA using specific '102' primers in different instruments and ROX modes. (A) qPCR performance under ABI StepOne Plus no ROX mode; (B) qPCR performance under ABI StepOne Plus high ROX mode; (C) qPCR performance under ABI QuantStudio 3 low ROX mode; (D) qPCR performance under Quantagene q225.
2. Product comparisons
- Amplification plot of E. coli gDNA using specific '102' primers and qPCR master mixes from different manufacturers. Curves indicate ABclonal qPCR master mix yields higher signal with higher resolution.
3. Results after freeze-thaw cycles
- Amplification plot of E. coli gDNA using specific '102' primers. ABclonal 2X Universal SYBR Green Fast qPCR Mix can withstand 100 freeze-thaw cycles without significant activity loss.
4. Demonstrated Specificity
- Amplification curve (A) and melt curve (B) are plots of E. coli gDNA using ABclonal 2X Universal SYBR Green Fast qPCR Mix and specific “120” primers over a 8-log-range; (C) A standard curve with 99% PCR reaction efficiency and correlation coefficient value (R2) 0.999.
5. Strong performance with multiple genes of various species
- (A) Amplification plot of E. coli gDNA using specific '102' primers. The high sensitivity and broad dynamic range of ABclonal 2X Universal SYBR Green Fast qPCR Mix enables accurate quantification of a 8-log-range template test; (B) Single melt curves indicate specific amplification and reliable results; (B) qPCR targeting GAPDH, beta-Actin and Ywhaz was performed using ABclonal 2X Universal SYBR Green Fast qPCR Mix over Mouse cDNA. (C) qPCR targeting GAPDH, FARP and TGF was performed using ABclonal 2X Universal SYBR Green Fast qPCR Mix over Rabbit cDNA.
Documents
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