Mammalian Protein Expression

Services / Protein Services / Mammalian Expression

The mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay. At ABclonal we produce proteins in both transient and stable formats using the HEK293 or the CHO cells. We utilize proprietary expression vectors to drive the high levels of the expression and regularly return >10mg/L of the recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for the large-scale production yielding grams of the desired protein.

Mammalian Cell Expression Flowchart

Highlights

  • High success rate

    The success rate is up to 90%.

  • Optional Expression Systems
    • Transient expression, recombinant antibody expression, and preparation, stable cell line expression service, etc.
    • Transient expression: ABclonal offers three 293-suspension cells lines and three CHO suspension cell lines that cultured in serum-free medium. At the same time, our independent, intelligent property rights of the highly efficient expression vectors can raise the yield to 3~6 fold.
    • Stable cell line development: The independent, intelligent property rights of a reformed CHO-K1 cell line is used for protein and antibody production.
  • Large-scale Production

    The serum-free culture cells can reach up to 200L per batch.

  • The Whole Project Service

    From gene to protein: Design and optimize the gene, virus production, express and optimize the protein, process the protein post-purification, deal with the data and write the experimental report for the customer.

Transient Expression Service

Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization & subclone

 

2 Weeks
Protein Expression Test
  • Endotoxin-free plasmid extraction and quality control
  • Small-scale transient
  • Optimize the expression condition, SDS-PAGE/WB identification, 80 mL amplification and purification test on the optimum conditions
  • Tag-free test (if required)

 

3-4 Weeks
Large-scale Expression & Purification
  • Endotoxin-free plasmid extraction in a large amount and quality control
  • Large-scale transient and expression
  • Protein affinity purification (or other purification methods)
  • Tag-free protein (if required)
  • Quality Control
  3-5 Weeks

Stable Cell Line Construction Service

Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization & subclone

 

2 Weeks
Stable Cell Line Development
  • Endotoxin-free plasmid extraction and quality control
  • Small-scale transient (293 & CHO), expression test, SDS-PAGE/WB identification, pressure screening, cell subclone, expression, and adaption evaluate under the serum-free circumstance, build the library of the cell line.

 

2-4 Weeks
Large-scale Expression & Purification
  • Protein affinity purification (or other purification methods)
  • Tag-free protein (if required)
  • Quality Control
  3-5 Weeks
Quote
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.