We utilize proprietary expression vectors to achieve high levels of expression and regularly return >10mg/L of the recombinant protein.
The mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay.
At ABclonal, we produce proteins in both transient and stable formats using the HEK293 or the CHO cells. We utilize proprietary expression vectors to drive the high levels of the expression and regularly return >10mg/L of the recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for the large-scale production yielding grams of the desired protein.
Optional Expression Systems — Transient expression, recombinant antibody expression, and preparation, stable cell line expression service, etc. learn more
Transient expression: ABclonal offers three 293-suspension cells lines and three CHO suspension cell lines that cultured in serum-free medium. At the same time, our independent, intelligent property rights of the highly efficient expression vectors can raise the yield to 3~6 fold.
Stable cell line development: The independent, intelligent property rights of a reformed CHO-K1 cell line is used for protein and antibody production.
Service Name | Service Content | Timeline |
---|---|---|
Plasmid Construction |
|
2 Weeks |
Protein Expression Test |
|
3-4 Weeks |
Large-scale Expression & Purification |
|
3-5 Weeks |
Service Name | Service Content | Timeline |
---|---|---|
Plasmid Construction |
|
2 Weeks |
Stable Cell Line Development |
|
2-4 Weeks |
Large-scale Expression & Purification |
|
3-5 Weeks |