Mammalian Protein Expression

We utilize proprietary expression vectors to achieve high levels of expression and regularly return >10mg/L of the recombinant protein.

The mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay.

At ABclonal, we produce proteins in both transient and stable formats using the HEK293 or the CHO cells. We utilize proprietary expression vectors to drive the high levels of the expression and regularly return >10mg/L of the recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for the large-scale production yielding grams of the desired protein.

Mammalian Cell Expression Flowchart

Highlights

  • High Success Rate — The success rate is up to 90%.

  • Optional Expression Systems — Transient expression, recombinant antibody expression, and preparation, stable cell line expression service, etc.

  • Large-Scale Production — The serum-free culture cells can reach up to 200L per batch.
  • One-Stop Service — From gene design and optimization, virus production, protein expression, protein post-purification, to manage data and experimental report.

ptional Expression Systems

Transient Expression Service
Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization & subclone
 2 Weeks
Protein Expression Test
  • Endotoxin-free plasmid extraction and quality control
  • Small-scale transient
  • Optimize the expression condition, SDS-PAGE/WB identification, 80 mL amplification and purification test on the optimum conditions
  • Tag-free test (if required)
 3-4 Weeks
Large-scale Expression & Purification
  • Endotoxin-free plasmid extraction in a large amount and quality control
  • Large-scale transient and expression
  • Protein affinity purification (or other purification methods)
  • Tag-free protein (if required)
  • Quality Control
 3-5 Weeks
Stable Cell Line Construction Service
Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization & subclone
 2 Weeks
Stable Cell Line Development
  • Endotoxin-free plasmid extraction and quality control
  • Small-scale transient (293 & CHO), expression test, SDS-PAGE/WB identification, pressure screening, cell subclone, expression, and adaption evaluate under the serum-free circumstance, build the library of the cell line.
 2-4 Weeks
Large-scale Expression & Purification
  • Protein affinity purification (or other purification methods)
  • Tag-free protein (if required)
  • Quality Control
 3-5 Weeks
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