Mammalian Protein Expression

The mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay. At ABclonal we produce proteins in both transient and stable formats using the HEK293 or the CHO cells. We utilize proprietary expression vectors to drive the high levels of the expression and regularly return >10mg/L of the recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for the large-scale production yielding grams of the desired protein.

Highlights

---

• High success rate

  The success rate is up to 90%.

• Optional Expression Systems

  • Transient expression, recombinant antibody expression, and preparation, stable cell line expression service, etc.
  • Transient expression: ABclonal offers three 293-suspension cells lines and three CHO suspension cell lines that cultured in serum-free medium. At the same time, our independent, intelligent property rights of the highly efficient expression vectors can raise the yield to 3~6 fold.
  • Stable cell line development: The independent, intelligent property rights of a reformed CHO-K1 cell line is used for protein and antibody production.

• Large-scale Production

  The serum-free culture cells can reach up to 200L per batch.

• The Whole Project Service

  From gene to protein: Design and optimize the gene, virus production, express and optimize the protein, process the protein post-purification, deal with the data and write the experimental report for the customer.

Transient Expression Service

---

Service Name	Service Content	Timeline
Plasmid Construction	Gene synthesis with codon optimization & subclone		2 Weeks
Protein Expression Test	Endotoxin-free plasmid extraction and quality control Small-scale transient Optimize the expression condition, SDS-PAGE/WB identification, 80 mL amplification and purification test on the optimum conditions Tag-free test (if required)		3-4 Weeks
Large-scale Expression & Purification	Endotoxin-free plasmid extraction in a large amount and quality control Large-scale transient and expression Protein affinity purification (or other purification methods) Tag-free protein (if required) Quality Control		3-5 Weeks

Stable Cell Line Construction Service

---

Service Name	Service Content	Timeline
Plasmid Construction	Gene synthesis with codon optimization & subclone		2 Weeks
Stable Cell Line Development	Endotoxin-free plasmid extraction and quality control Small-scale transient (293 & CHO), expression test, SDS-PAGE/WB identification, pressure screening, cell subclone, expression, and adaption evaluate under the serum-free circumstance, build the library of the cell line.		2-4 Weeks
Large-scale Expression & Purification	Protein affinity purification (or other purification methods) Tag-free protein (if required) Quality Control		3-5 Weeks