Mammalian Protein Expression Service

Mammalian cells are the preferred expression platform for proteins requiring fully mammalian post-translational modifications. As such, they are commonly used for the production of antibodies and therapeutic proteins. They are also preferred when the downstream application for a recombinant protein is in a functional mammalian cell-based assay. At ABclonal we produce proteins in both transient and stable formats using HEK293 or CHO cells. We utilize proprietary expression vectors to drive high levels of expression and regularly return >10mg/L of recombinant protein. Our superior capacity allows us to conduct tens of transient expression projects simultaneously and our serum-free suspension culture format allows for large-scale production yielding grams of the desired protein.

Mammalian Cell Expression Flowchart

Highlights


  • High success rate

    The success rate is up to 90%.

  • Optional expression systems

    Transient expression, recombinant antibody expression and preparation, stable cell line expression service, etc.

    • a.Transient expression: ABclonal offers three 293 suspension cells lines and three CHO suspension cell lines which cultured in serum free medium, and our independent intelligent property rights of highly efficient expression vectors can raise the yield to 3~6 fold.
    • b.Stable cell line development: The independent intelligent property rights of a reformed CHO-K1 cell line is used for protein and antibody production.
  • Large-scale production

    The serum-free culture cells can reach up to 200L per batch.

  • One-stop service

    From gene to protein.Design and optimize gene, transient & stable cell line construction, recombinant antibody production and management of data and report.

Transient Expression Service


Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization& subclone

 

3-5 Weeks
Protein Expression Test
  • Endotoxin-free plasmid extraction and quality control
  • Small scale transient
  • Optimize the expression condition,SDS-PAGE/WB identification ,80ml amplification and purification test on the optimum conditions
  • Tag-free test (if required)

 

3-4 Weeks
Large-scale expression & purification
  • Endotoxin-free plasmid extraction in a large amount and quality control
  • Large scale transient and expression
  • Protein affinity purification(or other purification method)
  • Tag-free protein (if required)
  • QC the purified protein
1-10L 3-5 Weeks
10-20L 4-6 Weeks
20-50L 5-8 Weeks
Above 50L 7-14 Weeks

Stable Cell Line Construction Service


Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization& subclone

 

3-5 Weeks
Stable Cell Line Development
  • Endotoxin-free plasmid extraction and quality control
  • Small scale transient(293&CHO), expression test,SDS-PAGE/WB identification, pressure screening ,cell subclone,expression and adaption evaluate under serum free circumstance,bulid library of cell line.

 

2-4 Weeks
Large-scale expression & purification
  • Protein affinity purification(or other purification method)
  • Tag-free protein (if required)
  • QC the purified protein
1-10L 3-5 Weeks
10-20L 4-6 Weeks
20-50L 5-8 Weeks
Above 50L 7-14 Weeks

Note:

QC: SDS-PAGE/WB identification of the purified protein,MS identification(if required).

All service timelines are approximate. In most cases, we finish the custom project within the appointed time. However, optional steps might prolong the deliver timeline.

Custom protein service is charged step by step and save your valuable research costs.

Protein Service Quotation Request Form
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 86 Cummings Park, Woburn, Massachusetts.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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