When the projects are finished, we will send you purified antibodies free samples for your endogenous test. If you can supply the cDNA of the projects, we can offer you a free 100UL sample. If not, we will also offer you a free 50UL sample.
At last, if you're satisfied with our sample, we can offer you the rest of the product in 2 ways:
In addition, if you are not satisfied with the sample, please send back your testing results and we charge you nothing.
For other species,not within the specified range, you can turn to our custom antibody service.
For other research fields(not within the specified range), you can apply according to the provisions.We guarantee that 80% of the applications in our specified range will be approved.
Our projects start from preparation of prokaryotically-expressed antigen, we will choose the best rabbits to do the immunization.
ABclonal possesses a large cDNA bank which contains over 20,000 human genes. If we fail to supply the cDNA or you prefer to immunize mouse or rat, please kindly send us the plasmids which contain the cDNA. If you need antibodies produced by peptide or modified-peptide, please refer to our common custom polyclonal antibody preparation service.
ABclonal's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.
The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).We guarantee that a single antigen will be injected into both animals.
|Control Serum Collection||Day 0||Pre-immune bleed (5mL per rabbit)|
|Primary Injection||Day 1||Immunize with 0.50mg of antigen in CFA, 10 SQ sites|
|1st Booster||Day 14||Boost with 0.25mg of antigen in IFA, 4 SQ sites|
|2nd Booster||Day 28||Boost with 0.25mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 35||Bleed (~25mL per rabbit)|
|3rd Booster||Day 42||Boost with 0.25mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 56, 58||Two bleeds (~50mL total per rabbit)|
|ELISA Test||Day 60||
ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
|Purification||Optional||6-10 mg purified antibodies|