Free Trial Custom Polyclonal Antibody

Services / Antibody Services / Free Trial Services

Our Vivarium Is Capable Of Hosting 40,000 Rabbits Over The Course Of The Year. This Allows Us To Provide Free Custom Antibody Preparation Services And Multiple Types Of Partnerships For Our Customers Around The World.

Ways to get our service

If the proteins you are interested in are included in our free-trial domain and are from humans, mice, or rats, please just send us an email that includes the name of the proteins.

If the proteins are out of our free projects domain, that's OK. Just tell us the name of the proteins and we will evaluate the projects and see whether we can still offer you the free custom services.

Ways to get your products

When the projects are finished, we will send you free samples of the purified antibodies for your endogenous tests. If you can supply the cDNA of the projects, we can offer you a free, 100 μl sample. If not, we can still offer you a free, 50 μl sample.

If you are satisfied with our samples, we can send you the rest of the products in 2 ways:

  • Buy our standard-sized package at catalog price
  • Buy our large, custom antibodies package at catalog price

If you are not satisfied with the sample, please send back your test results and we will not charge you.

Why choose the ABclonal free custom antibody?

No risks at all. If we fail, you can choose to terminate or reactivate the project.

Recombinant protein-antigen. Higher possibility to conduct Co-IP, ChIP and IF experiments.

Wide range of proteins. Most proteins of humans, mice, and rats are all included in the free custom antibody preparation service.

Our specified research fields

For other species not within the specified range, you can turn to our custom antibody service.

Our specified research fields

DNA Strand Crosslink Repair

  • Angiogenesis
  • Apoptosis
  • Autophagy
  • Centrosomal Protein
  • DNA Damage/Repair
  • Epigenetics & Nuclear Function
  • Ion Channel Regulation
  • Mitochondrial Biogenesis
  • Neurodegenerative Diseases
  • RNA-Binding Protein
  • Transcription Factor
  • Ubiquitin-Protein
  • Hedgehog Signaling Pathway
  • Hippo Signaling Pathway
  • Jak-Stat Signaling Pathway
  • NF-kB Signaling Pathway
  • P38 MAPK Signaling Pathway
  • TGFB-Smad Signaling Pathway
  • Toll-Like Receptor Signaling Pathway
  • Wnt/Notch Signaling Pathway

For other research fields (not within the specified range), you can contact us for customization options. We guarantee that 80% of the applications in our specified range will be approved.

Modes for free custom antibody preparation

Our projects start from the preparation of prokaryotically-expressed antigens. We will choose the best rabbits to immunize.

ABclonal possesses a large cDNA bank which contains over 20,000 human genes. If we fail to supply the cDNA or you prefer to immunize mice or rats, please kindly send us the plasmids which contain the cDNA. If you need antibodies produced by peptides or modified peptides, please refer to our standard custom polyclonal antibody preparation service.

ABclonal's standard protocol for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option and a popular choice among researchers.

The protocol uses two New Zealand White rabbits that are Specific-Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA). We guarantee that the same antigen will be injected into both animals

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5 mL per rabbit)
Primary Injection Day 1 Immunize with 0.50 mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (-25 mL per rabbit)
3rd Booster Day 42 Boost with 0.25 mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (-50 mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online)
Verify disposition of rabbits
Decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Note: After Day 35, the scheduled days in this protocol are approximations and are dependent on the titer test.
  1. For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  2. Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  3. Please send your samples to ABclonal, 86 Cummings Park Dr, Woburn, MA 01801, United States.