Our vivarium is capable of hosting 40,000 rabbits over the course of the year. This allows us to provide free custom antibody preparation service and multiple types of partnerships for our customers all over the world.

Ways to get our service

If the proteins you're interested in belong to the domain and species such as human,mouse or rat we have covered, all you need to do is just sending an Email to tell us the name of the protein.

If the proteins are out of our free projects domain, it's OK. Just tell us the name of the proteins and we will evaluate the projects and tell you whether we can still offer you a free custom service or not.


Ways to get your products

When the projects are finished, we will send you purified antibodies free samples for your endogenous test. If you can supply the cDNA of the projects, we can offer you a free 100UL sample. If not, we will also offer you a free 50UL sample.

At last, if you're satisfied with our sample, we can offer you the rest of the product in 2 ways:

  • Buy our standard size package in catalog price
  • Buy our large package in custom antibodies catalog price

In addition, if you are not satisfied with the sample, please send back your testing results and we charge you nothing.

Why choose the ABclonal free custom antibody?

No risks at all. If we fail, you can choose to terminate or reactivate the project.

Recombinant protein-antigen. Higher possibility to conduct Co-IP, ChIP and IF experiments.

Wide range of proteins. Most proteins of human, mouse and rat are all involved in free custom antibody preparation service.


Our specified research fields

For other species,not within the specified range, you can turn to our custom antibody service.

Our specified research fields


DNA Strand Crosslink Repair

  • Angiogenesis
  • Apoptosis
  • Autophagy
  • Centrosomal Protein
  • DNA Damage/Repair
  • Epigenetics & Nuclear Function
  • Ion Channel Regulation
  • Mitochondrial Biogenesis
  • Neurodegenerative Diseases
  • RNA Binding Protein
  • Transcription Factor
  • Ubiquitin-Protein
  • Hedgehog Signaling Pathway
  • Hippo Signaling Pathway
  • Jak-Stat Signaling Pathway
  • NF-kB Signaling Pathway
  • P38 MAPK Signaling Pathway
  • TGFB-Smad Signaling Pathway
  • Toll-Like Receptor Signaling Pathway
  • Wnt/Notch Signaling Pathway

For other research fields(not within the specified range), you can apply according to the provisions.We guarantee that 80% of the applications in our specified range will be approved.

Modes for Free custom antibody preparation

Our projects start from preparation of prokaryotically-expressed antigen, we will choose the best rabbits to do the immunization.

ABclonal possesses a large cDNA bank which contains over 20,000 human genes. If we fail to supply the cDNA or you prefer to immunize mouse or rat, please kindly send us the plasmids which contain the cDNA. If you need antibodies produced by peptide or modified-peptide, please refer to our common custom polyclonal antibody preparation service.

ABclonal's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).We guarantee that a single antigen will be injected into both animals.

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5mL per rabbit)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (~25mL per rabbit)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (~50mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Note: After Day 35, the Scheduled days in this protocol are approximate. They depend on the titer test.
Quote
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to ABclonal, 86 Cummings Park, Woburn, Massachusetts.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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