When the projects are finished, we will send you free samples of the purified antibodies for your endogenous tests. If you can supply the cDNA of the projects, we can offer you a free, 100 μl sample. If not, we can still offer you a free, 50 μl sample.
If you are satisfied with our samples, we can send you the rest of the products in 2 ways:
If you are not satisfied with the sample, please send back your test results and we will not charge you.
For other species not within the specified range, you can turn to our custom antibody service.
For other research fields (not within the specified range), you can contact us for customization options. We guarantee that 80% of the applications in our specified range will be approved.
Our projects start from the preparation of prokaryotically-expressed antigens. We will choose the best rabbits to immunize.
ABclonal possesses a large cDNA bank which contains over 20,000 human genes. If we fail to supply the cDNA or you prefer to immunize mice or rats, please kindly send us the plasmids which contain the cDNA. If you need antibodies produced by peptides or modified peptides, please refer to our standard custom polyclonal antibody preparation service.
ABclonal's standard protocol for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option and a popular choice among researchers.
The protocol uses two New Zealand White rabbits that are Specific-Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA). We guarantee that the same antigen will be injected into both animals
|Control Serum Collection||Day 0||Pre-immune bleed (5 mL per rabbit)|
|Primary Injection||Day 1||Immunize with 0.50 mg of antigen in CFA, 10 SQ sites|
|1st Booster||Day 14||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|2nd Booster||Day 28||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 35||Bleed (-25 mL per rabbit)|
|3rd Booster||Day 42||Boost with 0.25 mg of antigen in IFA, 4 SQ sites|
|Serum Collection||Day 56, 58||Two bleeds (-50 mL total per rabbit)|
|ELISA Test||Day 60||
ELISA titration (results available online)
Verify disposition of rabbits
Decide to continue or terminate
|Purification||Optional||6-10 mg purified antibodies|