Flow cytometry is a powerful experimental tool with applications in myriad bioscience fields including immunology and cancer biology. With huge advances over the past decades and greater accessibility to flow technology, ABclonal is proud to provide high-quality, convenient flow cytometry-validated antibodies to accommodate your unique research needs!
The only direct fluorescent conjugated recombinant rabbit monoclonal flow cytometry primary antibody on the market:
Maximize flexibility:
Flow cytometry is a common laboratory method used to analyze characteristics of cell populations, usually from blood or other body fluids. Among the applications of flow cytometry data are:
• Cell counting and identification
• Cell sorting for subsequent experimentation
• Diagnostics via the detection of biomarkers
Flow cytometry can be used to evaluate disease states that includes viral and bacterial infections, cancer, and autoimmune diseases. ABflo® products are specially formulated to ensure accurate positive identification of common cell surface and intracellular markers. All ABflo® offerings are recombinant rabbit monoclonal antibodies generated using our SMab™ technology, ensuring batch consistency and experimental reproducibility with an optimized target-specific reagent!
ABflo® antibodies are directly conjugated to superior fluorescent dyes that provide stable signals and reliable detection for a wide range of common markers on immune cells and other cell types. Available standard in the green (ABflo® 488) and red (ABflo® 647) channels, ABflo® provides the versatility to explore additional targets in as many other channels as your flow procedure allows. Secondary antibodies are also available for wavelengths across a wide spectrum to increase the scope of your experiment.
ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb (A22773)
Flow cytometry: 1X10^6 THP-1 cells were surface stained with ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb(A22773, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line).Non-fluorescently stained THP-1 cells was used as blank control (red line).
Flow cytometry: 1X10^6 K562 cells(negative control) were surface stained with ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb(A22773, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line).Non-fluorescently stained K562 cells was used as blank control (red line).
Flow cytometry:1X10^6 THP-1 cells were surface stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or ABflo® 488 Rabbit anti-Human/Monkey CD4 mAb(A22773, 2 μg/mL, right).
ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb (A23009)
Flow cytometry: 1X10^6 293F cells(negative control) were surface stained with ABflo® 647 Rabbit anti-Monkey CD19 mAb(A23009, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line).Non-fluorescently stained 293F cells was used as blank control (red line).
Flow cytometry: 1X10^6 Raji/Daudi cells were surface stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line).Non-fluorescently stained Raji cells was used as blank control (red line).
Flow cytometry:1X10^6 Raji/Daudi cells were surface stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009, 2 μg/mL, right).
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