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Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (DET) (RM17575)


ABclonal:Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (DET)


Product nameRabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (DET)
Catalog No.RM17575
CatagoryPrimary antibody
DescriptionCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
ImmunogenE.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419(Gly335Ala)
Accession #QHD43423.2
PurirtyAffinity purification
Ig TypeRabbit IgG
Endotoxin Level< 1.0 EU/μg of the protein by L AL method.
FormulationSupplied as a 0.2um filtered solution in PBS, pH 7.4.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.

Avoid repeated freeze-thaw cycles.


SARS-COV-2 Nucleocapsid Sandwich Immunoassay

 Recommended ConcentrationSample
ELISA Capture1-4ug/mLRabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(CAP)(Cat. No. RM17574)
ELISA Detection0.05-0.2ug/mLRabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(DET)(Cat. No. RM17575)
Standard1.56-100pg/mLRecombinant SARS-COV-2 Nucleocapsid Protein with His tag(Cat. No. RP01264)

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.

Cat. No. RM17574, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .

* For research use only. Not for therapeutic or diagnostic purposes.

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