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Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)

ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)
ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)
ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)

Overview

Product name Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP)
Catalog No. RM17574
Catagory Elisa Antibody Kit
Application ELISA
Description Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Immunogen HK293 derived SARS-COV-2 Nucleocapsid
Ser2-Ala419
Accession #YP_009724397.2
Purirty Affinity purification
Ig Type Rabbit IgG
Endotoxin Level < 1.0 EU/μg of the protein by LAL method.
Formulation Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4.
CrossReactivity Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# RP01281)
No cross-reactivity in ELISA with
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative:0.1%Braveds MB-1

Avoid repeated freeze-thaw cycles.

Application

SARS-COV-2 Nucleocapsid Sandwich Immunoassay

 Recommended ConcentrationSample
ELISA Capture1-4ug/mLRabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(CAP)(Cat. No. RM17574)
ELISA Detection0.05-0.2ug/mLRabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(DET)(Cat. No. RM17575)
Standard1.56-100pg/mLRecombinant SARS-COV-2 Nucleocapsid Protein with His tag(Cat. No. RP01264)
ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)}

Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)}

Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)

No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.
ABclonal:Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)}

Western blot - Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) (RM17574)

Cat. No. RM17574, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .

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