Product name | Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb (CAP) |
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Catalog No. | RM17574 |
Catagory | Elisa Antibody Kit |
Application | ELISA |
Description | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
Immunogen | HK293 derived SARS-COV-2 Nucleocapsid Ser2-Ala419 Accession #YP_009724397.2 |
Purirty | Affinity purification |
Ig Type | Rabbit IgG |
Endotoxin Level | < 1.0 EU/μg of the protein by LAL method. |
Formulation | Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4. |
CrossReactivity | Has cross-reactivity in ELISA with SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# RP01281) No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein HCoV-229E Nucleoprotein protein |
Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative:0.1%Braveds MB-1 Avoid repeated freeze-thaw cycles. |
SARS-COV-2 Nucleocapsid Sandwich Immunoassay
Recommended Concentration | Sample | |
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ELISA Capture | 1-4ug/mL | Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(CAP)(Cat. No. RM17574) |
ELISA Detection | 0.05-0.2ug/mL | Rabbit anti-SARS-CoV-2 Nucleocapsid Protein mAb(DET)(Cat. No. RM17575) |
Standard | 1.56-100pg/mL | Recombinant SARS-COV-2 Nucleocapsid Protein with His tag(Cat. No. RP01264) |
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* For research use only. Not for therapeutic or diagnostic purposes.